Screening for Free Radical Scavenging Activities of Extracts from Coccinia grandis and Centella asiatica

Main Article Content

Poungrat Pakdeechote
Upa Kukongviriyapan
Veerapool Kukongviriyapan

Abstract

Background : Coccinia  grandis  and  Centella  asistica  are  tropical  vegetables  and  acclaimed  in  Thai  folk  medicind  for  treatment  of  various  diseases.  The  mechanisms  responsible  for  their  pharmacological  effects  remain  uncertain.  The  antioxidant  activities.  Are  proposed  to play  role  in  th  mechanism  of  action.

Objectives : To investigate  the  antioxidant  activities  of  C.grandis  and  C.asiatica.

Method :  Free  radical  scavenging  activity  was  assessed  by  1, 1 – Diphenyl – 2 picrylhydrazyl (DPPH)  assay.  Total  antioxidant  power  (FRAP)  assay.  Intracellular  oxygen  radicals  scavenging  effect  in  rats  peritoneal  macrophages  was  evaluated  by  using  fluorescent  dichlorofluorescein  probe.

Results: C.grandis  and  C.asiatica  extracts  showed  ability  to  scavenge  DPPH  radical  with  EC 50 values  of  164.78+5.63 and  172.33+9.13 ug/ml,  respectively.  It  appeared  that  their  potency  were  about  100 folds  less  than  that  of  ascorbic  acid  and  trolox.  Total  antioxidant  power  of  wxtracts,  as  assayed  by  FRAP  method  revealed  that  1 mg  of  C. Grandis  extract  had  reducing  power  comparable  to  ascorbic  acid  8.23 + 0.64 ug or FeCl2 11.4+0.92 ug.  In  addition,  both  plant  extracts  at  the  concentration  of  100, 300 ug/ma  and  tiron 10, 30 ug/ml  significantly  decreased  the  formation  of  oxygen  radicals  generated  in  rat  peritoneal  macrophages.

Conclusins : Although  both extracts  had  relatively  low  reducing  power  in  these  in  vitro  assays,  however,  thir  antioxidant  effects  as  shown  in  peritoneal  macrophages  may  be  of  significance  and  worthwhile  for  further  investigation.

Downloads

Download data is not yet available.

Article Details

How to Cite
1.
Pakdeechote P, Kukongviriyapan U, Kukongviriyapan V. Screening for Free Radical Scavenging Activities of Extracts from Coccinia grandis and Centella asiatica. SRIMEDJ [Internet]. 2013 Nov. 25 [cited 2022 Oct. 6];18(2):78-84. Available from: https://li01.tci-thaijo.org/index.php/SRIMEDJ/article/view/14445
Section
Original Articles