The Effect of Chrysin on Memory Impairments in Aging Rats Induced by D-galactose

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Ram Prajit
Nataya Sritawan
Kornrawee Suwannakot
Salinee Naewla
Anusara Aranarochana
Apiwat Sirichoat
Wanassanan Pannangrong
Jariya Umka Welbat


Background and Objective:         Brain aging is one of aging problems that affects to the neural stem cells in the subgranular zone (SGZ) of the dentate gyrus (DG) in hippocampus, which exhibits cognitive impairments. D-galactose (D-gal) could induces neuronal apoptosis caused by oxidative stress and inflammation. It is used in several brain aging studies. Chrysin, one of flavonoid, has many neuroprotective effects that can improve memory. This study investigated the effects of chrysin on memory impairments in aging rats induced by D-gal.

Methods:             Male Sprague Dawley rats were divided into 6 groups; control, D-gal, chrysin 10, chrysin 30, D-gal + chrysin 10 and D-gal + chrysin 30 groups. D-gal (50 mg/kg) was administrated by intraperitoneal (i.p.) injection. Chrysin (10 and 30 mg/kg) was administrated by oral gavage. Both of D-gal and chrysin were administrated for 8 weeks. After treatments, the body weight and locomotor activity were determined. The memories were evaluated using novel object location (NOL) and novel object recognition (NOR) tests.

Results:                The results showed that the body weight and locomotor activity did not significant differences among all groups. In the memory tests, control, chrysin 10, chrysin 30, D-gal + chrysin 10 and D-gal + chrysin 30 groups showed significant differences to discriminate the novel location and object in the NOL and NOR tests. In contrast, D-gal group showed no significant difference of discrimination in the both tests.

Conclusion:        This study demonstrates that D-gal induced memory impairments. However, chrysin could attenuated the memory impairments caused by D-gal.


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