Tangeretin Alleviates L-NAME Induced Vascular Dysfunction in Rats

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Chutamas Wunpathe
Putcharawipa Maneesai
Sarawoot Bunbupha
Thewarid Berkban
Parichat Prachaney
Upa Kukongviriyapan
Poungrat Pakdeechote


Background and Objective: Tangeretin is a flavonoid compound found in citrus fruits.  Several biological activities of tangeretin have been revealed including; anti-neurodegeneration, anti-inflammation, and anti-oxidation. This study was to investigate the effects of tangeretin on vascular function in L-NAME-treated rats. 

Methods: Rats were divided into 4 groups. Groups I; rats treated with L-NAME (40 mg/kg) in drinking water only, Groups II and III; rats treated with L-NAME together with tangeretin (15 and 30 mg/kg, respectively), Groups IV;  control rats were received only distilled water (n=7/each group). At the end of experiment, vascular responses to electrical field stimulation (EFS) and vasoactive agents in mesenteric vascular beds and aortic rings were evaluated.

Results: A significant increase in contractile response to electrical EFS was observed in the mesenteric vascular bed isolated from L-NAME-treated rats (p<0.05).  Tangeretin reduced the augmented response to EFS in L-NAME-treated rats comparing to those of untreated rats (p<0.05).  However, the contractile response to exogenous norepinephrine was not different among groups.  Vasorelaxation responses to acetylcholine (ACh) in the mesenteric vascular beds and aortic rings were significantly blunted in L-NAME-treated rats compared to control rats (p<0.05).  Treatment with tangeretin improved the vasorelaxation response to ACh in L-NAME-treated rats compared to untreated rats (p<0.05).  There was no significant difference in the

vasorelaxation responses to sodium nitroprusside among groups.

Conclusions: The results of this study indicated that tangeretin suppresses sympathetic nerve mediated contractile response and improves endothelium-dependent vasorelaxtion in L-NAME-treated rats.  


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