Syzygium gratum Extract Attenuates Renal Fibrosis in L-NAME Induced-Hypertensive Rats

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Sarawoot Bunbupha
Kwanjit Apaijit
Sariya Meephat
Putcharawipa Maneesai
Parichat Prachaney
Poungrat Pakdeechote


Background and Objectives: Syzygium gratum (SG) extract has antioxidant, anticancer and vascular protective activities. This study aimed to investigate the effect of SG extract on blood pressure and renal remodeling in Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME)-induced hypertensive rats.

Methods: Male Sprague-Dawley rats were administered with L-NAME (40 mg/kg/day) in drinking water for 5 weeks in order to induce hypertension. Hypertensive rats were treated with SG extract (300 mg/kg/day) or vehicle for the last 2 weeks. Systolic blood pressure and renal fibrosis were evaluated.

Results: Rats treated with L-NAME had high systolic blood pressure (187.8 ± 5.1 mmHg vs. 124.4 ± 2.8 mmHg) and increases in renal tubulointerstitial (3.8 ± 0.1 % vs. 2.1 ± 0.2 %) and corpuscular fibrosis (11.3 ± 1.1 % vs. 4.8 ± 0.4 %) compared with control rats (p<0.05). Treatment of hypertensive rats with SG extract significantly reduced systolic blood pressure (154.7 ± 2.7 mmHg), and alleviated renal tubulointerstitial and corpuscular fibrosis (2.4 ± 0.1 % and 5.5 ± 0.4 % respectively) (p<0.05).

Conclusion: Our results indicate that SG extract decreased blood pressure and alleviated renal fibrosis in L-NAME-induced hypertensive rats.


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1. Tozawa M, Iseki K, Iseki C, Kinjo K, Ikemiya Y, Takishita S. Blood pressure predicts risk of developing end-stage renal disease in men and women. Hypertension 2003; 41: 1341-5.
2. Martinez-Maldonado M. Role of hypertension in the progression of chronic renal disease. Nephrol Dial Transplant 2001; 16 (Suppl 1): 63-6.
3. Ribeiro MO, Antunes E, de Nucci G, Lovisolo SM, Zatz R. Chronic inhibition of nitric oxide synthesis. A new model of arterial hypertension. Hypertension 1992; 20: 298-303.
4. Zambrano S, Blanca AJ, Ruiz-Armenta MV, Miguel-Carrasco JL, Arevalo M, Mate A, et al. L-carnitine attenuates the development of kidney fibrosis in hypertensive rats by upregulating PPAR-gamma. Am J Hypertens 2014; 27: 460-70.
5. Silambarasan T, Manivannan J, Raja B, Chatterjee S. Prevention of cardiac dysfunction, kidney fibrosis and lipid metabolic alterations in l-NAME hypertensive rats by sinapic acid--Role of HMG-CoA reductase. Eur J Pharmacol 2016; 777: 113-23.
6. Ikeda H, Tsuruya K, Toyonaga J, Masutani K, Hayashida H, Hirakata H, et al. Spironolactone suppresses inflammation and prevents L-NAME-induced renal injury in rats. Kidney Int 2009; 75: 147-55.
7. Neergheen VS, Soobrattee MA, Bahorun T, Aruoma OI. Characterization of the phenolic constituents in Mauritian endemic plants as determinants of their antioxidant activities in vitro. J Plant Physiol 2006; 163: 787-99.
8. Kukongviriyapan U, Luangaram S, Leekhaosoong K, Kukongviriyapan V, Preeprame S. Antioxidant and vascular protective activities of Cratoxylum formosum, Syzygium gratum and Limnophila aromatica. Biol Pharm Bull 2007; 30: 661-6.
9. Stewart P, Boonsiri P, Puthong S, Rojpibulstit P. Antioxidant activity and ultrastructural changes in gastric cancer cell lines induced by Northeastern Thai edible folk plant extracts. BMC Complement Altern Med 2013; 13: 60.
10. Meephat S, Maneesai P, Bunbupha S, Prachaney P, Kukongviriyapan U, Pakdeechote P. Effect of Syzygium gratum extract on blood pressure and endothelium-dependent vasorelaxation in nitric oxide-deficient rats. Srinagarind Med J 2018; 33: 38-42.
11. Bunbupha S, Pakdeechote P, Kukongviriyapan U, Prachaney P, Kukongviriyapan V. Asiatic acid reduces blood pressure by enhancing nitric oxide bioavailability with modulation of eNOS and p47phox expression in L-NAME-induced hypertensive rats. Phytother Res 2014; 28: 1506-12.
12. Maggiorani D, Dissard R, Belloy M, Saulnier-Blache JS, Casemayou A, Ducasse L, et al. Shear Stress-Induced Alteration of Epithelial Organization in Human Renal Tubular Cells. PLoS One 2015; 10: e0131416.
13. Essig M, Friedlander G. Tubular shear stress and phenotype of renal proximal tubular cells. J Am Soc Nephrol 2003; 14 (Suppl 1): S33-5.
14. Zhao W, Chen SS, Chen Y, Ahokas RA, Sun Y. Kidney fibrosis in hypertensive rats: role of oxidative stress. Am J Nephrol 2008; 28: 548-54.
15. Zhang JJ, Bledsoe G, Kato K, Chao L, Chao J. Tissue kallikrein attenuates salt-induced renal fibrosis by inhibition of oxidative stress. Kidney Int 2004; 66: 722-32.
16. Karanovic D, Grujic-Milanovic J, Miloradovic Z, Ivanov M, Jovovic D, Vajic UJ, et al. Effects of Single and Combined Losartan and Tempol Treatments on Oxidative Stress, Kidney Structure and Function in Spontaneously Hypertensive Rats with Early Course of Proteinuric Nephropathy. PLoS One 2016; 11: e0161706.
17. Sugama I, Kohagura K, Yamazato M, Nakamura T, Shinzato T, Ohya Y. Superoxide dismutase mimetic, tempol, aggravates renal injury in advanced-stage stroke-prone spontaneously hypertensive rats. J Hypertens 2014; 32: 534-41.
18. Luo WM, Kong J, Gong Y, Liu XQ, Yang RX, Zhao YX. Tongxinluo Protects against Hypertensive Kidney Injury in Spontaneously-Hypertensive Rats by Inhibiting Oxidative Stress and Activating Forkhead Box O1 Signaling. PLoS One 2015; 10: e0145130.
19. Bureemas J, Kukongviriyapan V, Kukongviriyapan U, Senggunprai L, Jetsrisuparb A. Effects of Syzygium gratum on antioxidant system in β-thalassemia/Hb E patients. KKU Res J 2007; 7: 15-21.