Effect of Nelumbo nucifera Stamen Extract on Memory Deficits in Aβ-induced Rat

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Jenjiralai Phanphak
Chantana Boonyarat
Ploenthip Puthongking
Jariya Umka Welbat
Komsun Bunreungthong
Nutchareeporn Nillert
Wanassanun Pannangrong

Abstract

Background and Objectives:  Nelumbo nucifera (lotus) is a key plant in Thai traditional medicine that has anti-diabetic and anti-oxidant activities, and inhibits acetylcholinesterase enzyme. It is widely consumed as functional food. This study investigated the effect of N. nucifera stamen extract (NNSE) on memory deficit in amyloid beta (Aβ)-induced rat.


Methods: Forty adult male Sprague Dawley rats were divided into 5 groups, each group consisting of 8 rats as follows: Group 1, the normal control group. Group 2, the vehicle plus Aβ group (V+Aβ), received 0.5% sodium carboxymethyl cellulose, orally and was injected with amyloid-β via the lateral ventricles. Group 3, the vitamin C plus Aβ group (Vit C+Aβ), received vitamin C orally at 200 mg/kgBW., and was injected with Aβ via the lateral ventricles. Group 4, and 5, the NNSE plus Aβ groups (NNSE250+Aβ, NNSE500+Aβ), received NNSE orally at doses of 250, and 500 mg/kgBW, respectively, and were injected with Aβ via the lateral ventricles. All treatments were performed for 33 consecutive days. At day 21, Aβ (1-42) was injected via both sides of lateral ventricles. Ten days after Aβ1-42 injection, the rats were tested for locomotor activity and recognition memory by using the open field and novel object recognition tasks, respectively.


Results: The NNSE at dose of 500 mg/kg BW significantly increase the velocity and moving distance in locomotor activity in rats after induced with Ab. Moreover, NNSE at doses of 250 and 500 mg/kgBW significantly improve short-term recognition in cognitively impaired rats. These doses of NNSE also tend to improve long-term recognition in memory impaired rats.


Conclusions: NNSE improve memory impairments induced by Ab, especially short-term memory. Furthermore, the high doses of NNSE stimulates muscle function by increasing speed and movement distance.

Article Details

How to Cite
1.
Phanphak J, Boonyarat C, Puthongking P, Umka Welbat J, Bunreungthong K, Nillert N, Pannangrong W. Effect of Nelumbo nucifera Stamen Extract on Memory Deficits in Aβ-induced Rat . SRIMEDJ [Internet]. 2020 Jun. 4 [cited 2024 Mar. 29];35(3):261-5. Available from: https://li01.tci-thaijo.org/index.php/SRIMEDJ/article/view/243947
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Original Articles

References

1. Alzheimer’s Association. FDA-approved treatments for Alzheimer’s. [online] 2017 [cited May 25, 2019] Available from: https://www.alz.org/media/Documents/fda-approved-treatments-alzheimers-ts.pdf.
2. Nunomura A, Castellani RJ, Zhu X, Moreira PI, Perry G, Smith MA. Involvement of oxidative stress in Alzheimer disease. J Neuropathol Exp Neurol 2006; 65: 631-41.
3. Plant LD, Boyle JP, Smith IF, Peers C, Pearson HA. The production of amyloid β peptide is a critical requirement for the viability of central neurons. Neuroscience 2003; 23: 5531-5.
4. Hritcu L, Noumedem JA, Cioanca O, Hancianu M, Postu P, Mihasan M. Anxiolytic and antidepressant profile of the methanolic extract of Piper nigrum fruits in beta-amyloid (1–42) rat model of Alzheimer’s disease. Behav Brain Funct 2015; 11: 13-25.
5. Uddin S, Al Mamun A, Hossain S, Ashaduzzaman M, Noor AA, Hossain S, et al. Neuroprotective effect of Phyllanthus acidus L. on learning and memory impairment in scopolamine-induced animal model of dementia and oxidative stress: natural wonder for regulating the development and progression of Alzheimer’s disease. Adv Alzheimer Dis 2016; 5: 53-72.
6. Oh JH, Choi BJ, Chang MS, Park SK. Nelumbo nucifera semen extract improves memory in rats with scopolamine-induced amnesia through the induction of choline acetyltransferase expression. Neuro Sci Lett 2009; 461: 41-4.
7. Jitsaeng K, Sungthong B. Antioxidant Activity and Total Phenolic Contents of Various Parts from Two Cultivars of Nelumbo nucifera Gaertn. J Sci Technol MSU 2017; 36: 154-60.
8. Limwachiranon J, Huang H, Shi Z, Li L, Luo Z. Lotus Flavonoids and Phenolic Acids: Health Promotion and Safe Consumption Dosages. Compr Rev Food Sci Food Saf 2018; 17: 458-71.
9. Mukherjee PK, Mukherjee D, Maji AK, Rai S, Heinrich M. The sacred lotus (Nelumbo nucifera) –phytochemical and therapeutic profile. J Pharm Pharmacol 2009; 61: 407-22.
10. Sridhar KR, Bhat R. Lotus-a potential nutraceutical source. J Agri Tech 2007; 3: 143-55.
11. Paxinos G, Watson C. The rat brain in stereotaxic coordinates: hard cover edition. Elsevier: 2006.
12. Fu L, Liu C, Chen L, Lv Y, Meng G, Hu M, et al. Protective Effects of 1-Methylnicotinamide on Aβ 1–42-Induced Cognitive Deficits, Neuroinflammation and Apoptosis in Mice. J Neuroimmune Pharm 2019; 14: 401-12.
13. Hammond RS, Tull LE, Stackman RW. On the delay-dependent involvement of the hippocampus in object recognition memory. Neurobiol Learn Mem 2004; 82: 26-34.
14. Antunes M, Biala G. The novel object recognition memory: neurobiology, test procedure, and its
modifications. Cogn Process 2012; 13: 93-110.
15. Ennaceur A, Neave N, Aggleton JP. Spontaneous Object Recognition and Object Location Memory in Rats: The Effects of Lesions in the Cingulate Cortices, the Medial Prefrontal Cortex, the Cingulum Bundle and the Fornix. Exp Brain Res 1997; 113: 509-19.
16. Chen Z, Zhong C. Oxidative stress in Alzheimer’s disease. Neurosci Bull 2014; 30: 271–81.
17. Kumar A, Singh A. A review on mitochondrial restorative mechanism of antioxidants in Alzheimer’s disease and other neurological conditions. Front Pharmacol 2015; 6: 206-18.
18. Varadarajan S, Yatin S, Aksenova M, Butterfield DA. Review: Alzheimer’s Amyloid b-Peptide-Associated Free Radical Oxidative Stress and Neurotoxicity. J Struct Biol 2000; 130: 84-208.
19. Jung HA, Jung YJ, Yoon NY, Jeong DM, Bae HJ, Kim D-W et al. Inhibitory effects of Nelumbo nucifera leaves on rat lens aldose reductase, advanced glycation end products formation, and oxidative stress. Food Chem Toxicol 2008; 46: 3818-26.
20. The National Medicine Committee. The 2006 List of medicines from herbs.2nd ed. Bangkok: The Agricultural Co-operative Federation of Thailand; 2008.