Effect of Captopril on Erectile Dysfunction and Sperm Quality in Hypertensive Rats
Keywords:
Captopril; erectile dysfunction; L-NAME hypertensive rats; sperm; oxidative stressAbstract
Background and Objectives: Hypertension has been reported to be associated with erectile and testicular dysfunction. This study aimed to evaluate the role of the anti-hypertensive drug, captopril, on erectile and testicular function in L-NAME-induced hypertensive rats.
Methods: Male Sprague-Dawley rats were divided into 3 groups including control group received tap water, L-NAME group received L-NAME at dose 40 mg/kg/day for five weeks and captopril group received L-NAME and captopril at dose 5 mg/kg/day for the last two weeks. At the end of the study, systolic blood pressure, erectile response to nerve stimulation, sperm concentration, sperm motility, and oxidative stress markers were determined.
Results: L-NAME administration caused high blood pressure and decreased erectile response to nerve stimulation, reduced sperm concentrations, and sperm motility in rats. Moreover, the high level of vascular superoxide production, plasma, and tissue malondialdehyde, and low level of plasma nitric oxide metabolites were also observed in hypertensive rats compared to control rats (p<0.05). Treatment with captopril alleviated these reproductive alterations caused by L-NAME.
Conclusion: Captorpil improved erectile dysfunction and sperm quality in L-NAME-induced hypertensive rats. The possible mechanism may associate with its antihypertensive and antioxidant properties.
References
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20. Zhao S, Kang R, Deng T, Luo L, Wang J, Li E, et al. Comparison of two cannulation methods for assessment of intracavernosal pressure in a rat model. PLoS One 2018; 13: e0193543.
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22. Luangaram S, Kukongviriyapan U, Pakdeechote P, Kukongviriyapan V, Pannangpetch P. Protective effects of quercetin against phenylhydrazine-induced vascular dysfunction and oxidative stress in rats. Food Chem Toxicol 2007; 45: 448-455.
23. Verdon CP, Burton BA, Prior RL. Sample pretreatment with nitrate reductase and glucose-6-phosphate dehydrogenase quantitatively reduces nitrate while avoiding interference by NADP+ when the Griess reaction is used to assay for nitrite. Anal Biochem 1995; 224: 502-508.
24. Nakmareong S, Kukongviriyapan U, Pakdeechote P, Donpunha W, Kukongviriyapan V, Kongyingyoes B, et al. Antioxidant and vascular protective effects of curcumin and tetrahydrocurcumin in rats with L-NAME-induced hypertension. Naunyn Schmiedebergs Arch Pharmacol 2011; 383: 519-529.
25. Baylis C, Mitruka B, Deng A. Chronic blockade of nitric oxide synthesis in the rat produces systemic hypertension and glomerular damage. The Journal of clinical investigation 1992; 90: 278-281.
26. Gur S, Kadowitz PJ, Gurkan L, Chandra S, DeWitt SY, Harbin A, et al. Chronic inhibition of nitric coxide synthase induces hypertension and erectile dysfunction in the rat that is not reversed by sildenafil. BJU international 2010; 106: 78-83.
27. Akinyemi AJ, Adedara IA, Thome GR, Morsch VM, Rovani MT, Mujica LKS, et al. Dietary supplementation of ginger and turmeric improves reproductive function in hypertensive male rats. Toxicology Reports 2015; 2: 1357-1366.
28. Adedara IA, Alake SE, Adeyemo MO, Olajide LO, Ajibade TO, Farombi EO. Taurine enhances spermatogenic function and antioxidant defense mechanisms in testes and epididymis of L-NAME-induced hypertensive rats. Biomedicine & Pharmacotherapy 2018; 97: 181-189.
29. Mak IT, Freedman AM, Dickens BF, Weglicki WB. Protective effects of sulfhydryl-containing angiotensin converting enzyme inhibitors against free radical injury in endothelial cells. Biochem Pharmacol 1990; 40: 2169-2175.
30. Bartosz M, Kedziora J, Bartosz G. Antioxidant and prooxidant properties of captopril and enalapril. Free Radical Biology and Medicine 1997; 23: 729-735.
2. Muciaccia B, Pensini S, Culasso F, Padula F, Paoli D, Gandini L, et al. Higher clusterin immunolabeling and sperm DNA damage levels in hypertensive men compared with controls. Human reproduction 2012; 27: 2267-2276.
3. Gonzaga NA, do Vale GT, da Silva CB, Pinheiro LC, Leite LN, Carneiro FS, et al. Treatment with nitrite prevents reactive oxygen species generation in the corpora cavernosa and restores intracavernosal pressure in hypertensive rats. Nitric Oxide 2020; 94: 19-26.
4. Pasini AF, Garbin U, Nava MC, Stranieri C, Davoli A, Sawamura T, et al. Nebivolol decreases oxidative stress in essential hypertensive patients and increases nitric oxide by reducing its oxidative inactivation. Journal of hypertension 2005; 23: 589-596.
5. Wunpathe C, Maneesai P, Rattanakanokchai S, Bunbupha S, Kukongviriyapan U, Tong-Un T, et al. Tangeretin mitigates l-NAME-induced ventricular dysfunction and remodeling through the AT1R/pERK1/2/pJNK signaling pathway in rats. Food Funct 2020; 11: 1322-1333.
6. Yilmaz E, Kaya-Sezginer E, Yilmaz-Oral D, Cengiz T, Bayatli N, Gur S. Effects of hydrogen sulphide donor, sodium hydrosulphide treatment on the erectile dysfunction in L-NAME-induced hypertensive rats. Andrologia 2019; 51: e13240.
7. Kameni PM, Dzeufiet DPD, Bilanda DC, Mballa MF, Mengue NYS, Tchoupou TH, et al. Nymphaea lotus Linn. (Nymphaeaceae) Alleviates Sexual Disability in L-NAME Hypertensive Male Rats. Evid Based Complement Alternat Med 2019; 2019: 8619283.
8. Bunbupha S, Pakdeechote P, Kukongviriyapan U, Prachaney P, Kukongviriyapan V. Asiatic acid reduces blood pressure by enhancing nitric oxide bioavailability with modulation of eNOS and p47phox expression in L-NAME-induced hypertensive rats. Phytother Res 2014; 28: 1506-1512.
9. Kanthlal SK, Joseph J, Paul B, M V, P UD. Antioxidant and vasorelaxant effects of aqueous extract large cardamom in L-NAME induced hypertensive rats. Clin Exp Hypertens 2020; 42: 581-589.
10. Adedapo ADA, Ajayi AM, Ekwunife NL, Falayi OO, Oyagbemi A, Omobowale TO, et al. Antihypertensive effect of Phragmanthera incana (Schum) Balle on NG-nitro-L-Arginine methyl ester (L-NAME) induced hypertensive rats. J Ethnopharmacol 2020; 257: 112888.
11. Jan-On G, Sangartit W, Pakdeechote P, Kukongviriyapan V, Sattayasai J, Senaphan K, et al. Virgin rice bran oil alleviates hypertension through the upregulation of eNOS and reduction of oxidative stress and inflammation in L-NAME-induced hypertensive rats. Nutrition 2020; 69: 110575.
12. Chiangsaen P, Maneesai P, Kukongviriyapan U, Tong-un T, Ishida W, Prachaney P, et al. Tangeretin ameliorates erectile and testicular dysfunction in a rat model of hypertension. Reproductive Toxicology 2020; 96:1-10.
13. Wilson TW, Dubois MP. New drugs for the treatment of hypertension: where do they fit? Canadian Family Physician 1987; 33: 2583.
14. Rodriguez-Garcia JL, Villa E, Serrano M, Gallardo J, Garcia-Robles R. Prostacyclin: its pathogenic role in essential hypertension and the class effect of ACE inhibitors on prostaglandin metabolism. Blood Press 1999; 8: 279-284.
15. Maneesai P, Prasarttong P, Bunbupha S, Kukongviriyapan U, Kukongviriyapan V, Tangsucharit P, et al. Synergistic Antihypertensive Effect of Carthamus tinctorius L. Extract and Captopril in L-NAME-Induced Hypertensive Rats via Restoration of eNOS and AT(1)R Expression. Nutrients 2016; 8: 122.
16. Suzuki S, Sato H, Shimada H, Takashima N, Arakawa M. Comparative free radical scavenging action of angiotensin-converting enzyme inhibitors with and without the sulfhydryl radical. Pharmacology 1993; 47: 61-65.
17. Amat N, Amat R, Abdureyim S, Hoxur P, Osman Z, Mamut D, et al. Aqueous extract of dioscorea opposita thunb. normalizes the hypertension in 2K1C hypertensive rats. BMC Complement Altern Med 2014; 14: 36.
18. Mehta N, Sikka S, Rajasekaran M. Laboratory Forum: Rat as an Animal Model for Male Erectile Function Evaluation in Sexual Medicine Research. The Journal of Sexual Medicine 2008; 5: 1278-1283.
19. Wilkes N, White S, Stein P, Bernie J, Rajasekaran M. Phosphodiesterase-5 inhibition synergizes rho-kinase antagonism and enhances erectile response in male hypertensive rats. Int J Impot Res 2004; 187-194.
20. Zhao S, Kang R, Deng T, Luo L, Wang J, Li E, et al. Comparison of two cannulation methods for assessment of intracavernosal pressure in a rat model. PLoS One 2018; 13: e0193543.
21. Lu FJ, Lin JT, Wang HP, Huang WC. A simple, sensitive, non-stimulated photon counting system for detection of superoxide anion in whole blood. Experientia 1996; 52: 141-144.
22. Luangaram S, Kukongviriyapan U, Pakdeechote P, Kukongviriyapan V, Pannangpetch P. Protective effects of quercetin against phenylhydrazine-induced vascular dysfunction and oxidative stress in rats. Food Chem Toxicol 2007; 45: 448-455.
23. Verdon CP, Burton BA, Prior RL. Sample pretreatment with nitrate reductase and glucose-6-phosphate dehydrogenase quantitatively reduces nitrate while avoiding interference by NADP+ when the Griess reaction is used to assay for nitrite. Anal Biochem 1995; 224: 502-508.
24. Nakmareong S, Kukongviriyapan U, Pakdeechote P, Donpunha W, Kukongviriyapan V, Kongyingyoes B, et al. Antioxidant and vascular protective effects of curcumin and tetrahydrocurcumin in rats with L-NAME-induced hypertension. Naunyn Schmiedebergs Arch Pharmacol 2011; 383: 519-529.
25. Baylis C, Mitruka B, Deng A. Chronic blockade of nitric oxide synthesis in the rat produces systemic hypertension and glomerular damage. The Journal of clinical investigation 1992; 90: 278-281.
26. Gur S, Kadowitz PJ, Gurkan L, Chandra S, DeWitt SY, Harbin A, et al. Chronic inhibition of nitric coxide synthase induces hypertension and erectile dysfunction in the rat that is not reversed by sildenafil. BJU international 2010; 106: 78-83.
27. Akinyemi AJ, Adedara IA, Thome GR, Morsch VM, Rovani MT, Mujica LKS, et al. Dietary supplementation of ginger and turmeric improves reproductive function in hypertensive male rats. Toxicology Reports 2015; 2: 1357-1366.
28. Adedara IA, Alake SE, Adeyemo MO, Olajide LO, Ajibade TO, Farombi EO. Taurine enhances spermatogenic function and antioxidant defense mechanisms in testes and epididymis of L-NAME-induced hypertensive rats. Biomedicine & Pharmacotherapy 2018; 97: 181-189.
29. Mak IT, Freedman AM, Dickens BF, Weglicki WB. Protective effects of sulfhydryl-containing angiotensin converting enzyme inhibitors against free radical injury in endothelial cells. Biochem Pharmacol 1990; 40: 2169-2175.
30. Bartosz M, Kedziora J, Bartosz G. Antioxidant and prooxidant properties of captopril and enalapril. Free Radical Biology and Medicine 1997; 23: 729-735.
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Published
2020-10-16
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Chiangsaen P, Poasakate A, Pakdeechote P, Maneesai P. Effect of Captopril on Erectile Dysfunction and Sperm Quality in Hypertensive Rats. SRIMEDJ [Internet]. 2020 Oct. 16 [cited 2024 Apr. 20];35(6):726-31. Available from: https://li01.tci-thaijo.org/index.php/SRIMEDJ/article/view/247337
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