Effect of Chrysin on Methotrexate Chemotherapy Induced Reductions of Memory and Hippocampal Neurogenesis in Adult Rats

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Tanaporn Anosri
Soraya Kaewngam
Nataya Sritawan
Ram Prajit
Kornrawee Suwannakot
Apiwat Sirichoat
Anusara Aranarochana
Wanassanan Pannangrong
Jariya Umka Welbat


Background and objective: Methotrexate (MTX)is a chemotherapy drug that is the most widely used to protect against many malignancies. Several reports have shown that MTX can induce cognitive impairment. Chrysin is a natural flavonoid that is found in natural products. Previous studies have found that chrysin has neuroprotective and cognitive improving properties. Therefore, this study was designed to determine the protective effect of chrysin against MTX-induced memory impairments.

Methods: Seventy-two male Sprague Dawley rats were divided into 6 groups; control, MTX, chrysin 10, chrysin 30, chrysin 10+MTX and chrysin 30+MTX groups. The control group received saline and propylene glycol. Chrysin (10 and 30 mg/kg) was administered by oral gavage for 15 days. A single dose of MTX (75 mg/kg) was administered by intravenous injection on days 8 and 15. Three days after the end of drug administration, the body weight and locomotor activity were determined. The memories were evaluated using novel object location (NOL) and novel object recognition (NOR) tests.

Results: The data of all groups showed no significant differences in term of total exploration times. In both the NOL and NOR tests, the control, chrysin 10, chrysin 30, chrysin 10+MTX and chrysin 30+MTX groups could significantly discriminate between the familiar and novel location or object except the MTX group.

Conclusion: This study demonstrates that MTX induces memory impairment. In contrast, chrysin could improve memory impairment in rats received MTX.


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