Antibacterial Activity of Ethanolic Extract of Sida acuta Burm. F. Leaves Against Bacteria Isolated from Stone Matrices Antibacterial activity of ethanolic extract of Sida acuta Burm. F. leaves

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Nitis Smanthong
Ratree Tavichakorntrakool
Phitsamai Saisud
Patcharaporn Tippayawat
Aroonlug Lulitanond
Porntip Pinlaor
Arunrat Chaveerach
Jureerut Daduang
Nattaya Sae-ung
Patcharee Boonsiri


Background and Objective: The incidence of multidrug-resistant bacteria isolated from stone matrices of kidney stone patients has increased dramatically. Many plant extracts have been studied for antibacterial activity. Therefore, this study aimed to evaluate the antibacterial activity of the ethanolic extract of Sida acuta Burm. F. leaves (EESA) against bacteria isolated from kidney stone matrices.

Methods: The antibacterial activity of EESA was evaluated against two groups of bacteria, reference strains and clinical strains isolated from stone matrices by using the broth microdilution method. Each group of bacteria included three Gram-positive and three Gram-negative bacteria. Total flavonoid content of EESA was also determined.

Results: The minimum inhibitory concentrations of EESA against both reference and clinical strains for Gram-positive bacteria were in the range of 1-8 mg/mL whereas those of both groups for Gram-negative bacteria were in the range of 16-32 mg/mL. The best inhibitory activity of the EESA was observed against Staphylococcus saprophyticus. The EESA revealed the presence of flavonoids.

Conclusion: The EESA had antibacterial activity and flavonoids. The EESA may be developed as an alternative drug for the prevention and treatment of kidney stone disease with urinary tract infection especially S. saprophyticus.


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Smanthong N, Tavichakorntrakool R, Saisud P, Tippayawat P, Lulitanond A, Pinlaor P, Chaveerach A, Daduang J, Sae-ung N, Boonsiri P. Antibacterial Activity of Ethanolic Extract of Sida acuta Burm. F. Leaves Against Bacteria Isolated from Stone Matrices: Antibacterial activity of ethanolic extract of Sida acuta Burm. F. leaves. SRIMEDJ [Internet]. 2022 Apr. 28 [cited 2023 Feb. 7];37(2):179-82. Available from:
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