Asiatic Acid Alleviates Diabetic Kidney Injury Through Suppression of Renal Oxidative Stress
Keywords:
diabetic kidney injury, renal oxidative stress, asiatic acidAbstract
Background and Objective: Diabetes mellitus is a well-established risk factor for kidney injury. Chronic hyperglycemia in diabetes can potentially impair kidney function and structural changes through promotion of oxidative stress. The present study aimed to investigate the effect of asiatic acid (AA), an antioxidant on kidney injury and oxidative stress in diabetic rats.
Methods: Eight-week-old male Wistar rats weighing between 180 to 200 grams were induced to have type 2 diabetes by a single intraperitoneal injection of streptozotocin (STZ; 55 mg/kg) and nicotinamide (NA; 110 mg/kg). AA at doses of 10 or 20 mg/kg/day was administered intragastrically for 8 weeks. Metabolic changes in these rats were monitored during the experimental period. At the end of the experiment, kidney tissues and blood were collected from all rats for further experiments.
Results: Diabetic rats exhibited hyperglycemia, impaired insulin secretion, insulin insensitivity, an increased rate of kidney superoxide anion production. Moreover, diabetic rats also exhibited glomerular hypertrophy and glomerular ultrastructural changes. Treatment with AA, especially at a dose of 20 mg/kg/day, significantly attenuated insulin resistance, and kidney malondialdehyde (MDA) levels. AA also exerted a beneficial effect on renal protection by reducing glomerular hypertrophy and thickening of glomerular basement membranes.
Conclusions: AA alleviates kidney injury in diabetic rats through its glucose-lowering effect and antioxidant activity. These findings suggest the beneficial effects of AA as a potent antioxidant that could help prevent the progression of diabetic kidney complications.
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