Effects of Polycephalomyces Nipponicus Aqueous Extract on Mood and Motor Coordination in Acute Ethanol-Treated Mice
Keywords:
Polycepholomyces nipponicus, ethanol, mood disorder, motor impairment, miceAbstract
Background and Objective: Acute ethanol drinking leads to impaired motor coordination and judgment, which is one of the common causes of many accidents and deaths. Presently, many scientists have attracted medicinal plants containing polyphenols that have shown potential in alleviating the toxic and hangover effects of acute ethanol consumption. Polycephalomyces nipponicus, (P. nipponicus) an insect pathogenic fungus has demonstrated notable potential as a source of natural antioxidants and antimicrobial compounds and contains high total phenolic and flavonoid content. Therefore, the present study aimed to evaluate the effects of P. nipponicus extract on acute protective effects of ethanol-induced mood disorders and motor impairments in mice.
Methods: Male ICR mice were divided into 6 groups of 8 animals per group.: normal control, ethanol (2 g/kg), P. nipponicus extract (200, 600 mg/kg), and pretreated P. nipponicus, extract groups (200, 600 mg/kg). Before receiving ethanol, Mice were orally administered either water or P. nipponicus extract 200 or 600 mg/kg. One hour after, the mice were given either water or ethanol (2 g/kg). Thirty minutes following the second treatment, mice were subjected to exploratory test, tail suspension test (TST), rotarod test and footprint analysis.
Results: Ethanol treatment significantly decreased number of crossings, rearing and nose-poking in exploratory test indicating anxiety-like behavior, and increased immobility time in tail suspension test (TST) indicating depressive-like behavior. And then the rotarod test showed a decrease of time on the rod and an increase in gait abnormalities as observed in gait analysis, revealing the impairment of motor functions. P. nipponicus treatment, especially at 600 mg/kg, significantly alleviated the effects of ethanol in inducing mood disorders and motor impairments.
Conclusion: The results suggested that P. nipponicus extract could mitigate the acute effects of ethanol in inducing anxiety- and depressive-like behaviors and motor impairments.
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