Lipidomic Profiles of Cancer-Associated Fibroblasts with Cholangiocarcinoma Patients

Authors

  • Nisana Namwat Department of Systems Biosciences and Computational Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand-
  • Sirinya Sittirak Department of Systems Biosciences and Computational Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
  • Saikhim Sringan Medical Biochemistry and Molecular Biology Program, Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Thailand
  • Yingpinyapat Kittirat Regional Medical Sciences Center 2 Phitsanulok, Department of Medical Sciences, Ministry of Public Health, Thailand
  • Arporn Wangwiwatsin Department of Systems Biosciences and Computational Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
  • Poramate Klanrit Department of Systems Biosciences and Computational Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
  • Attapol Titapun Department of Surgery, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
  • Watcharin Loilome Department of Systems Biosciences and Computational Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

Keywords:

cancer-associated fibroblasts, cholangiocarcinoma, lipidomics

Abstract

Background and Objectives: Lipids is an essential component in tumor and they facilitate tumor growth and metastasis. Cancer-associated fibroblasts (CAFs) are defined as a tumor environment (TME) by which cells reside in stromal compartments supporting tumor progression. The lipid profile of CAFs, particularly in cholangiocarcinoma (CCA), have been unexploited. We aimed to investigate lipid profiles in CAFs of patients with CCA using lipidomic approach.

Methods: Three samples of CAFs were primarily isolated from tumor tissues of patients with CCA and subcultured in the laboratory. Lipid profiles in CAFs compared with normal fibroblasts (NFs) were analyzed by a non-targeted lipidomic analysis platform using ultra-high performance liquid chromatography-tandem mass spectroscopy (UHPLC-MS/MS).

Results: Forty-three lipid species in 3 groups were determined in CAFs including phosphatidylethanolamines, sphingomyelins and phosphatidylcholines. Those lipids presented in different levels between CAFs and NFs.

Conclusion: Lipids in CAFs are associated with metabolic adaptation that might support tumor progression and metastasis in CCA.  The lipid profiles in these CAFs have the potential to be applied in targeted therapies that disrupt metabolic interactions between CAFs and CCA cells.

Author Biographies

Nisana Namwat, Department of Systems Biosciences and Computational Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand-

Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

Sirinya Sittirak, Department of Systems Biosciences and Computational Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

Saikhim Sringan, Medical Biochemistry and Molecular Biology Program, Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Thailand

Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

Arporn Wangwiwatsin, Department of Systems Biosciences and Computational Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

Poramate Klanrit, Department of Systems Biosciences and Computational Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

Attapol Titapun, Department of Surgery, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

Watcharin Loilome, Department of Systems Biosciences and Computational Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

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Published

2025-02-26

How to Cite

1.
Namwat N, Sittirak S, Sringan S, Kittirat Y, Wangwiwatsin A, Klanrit P, Titapun A, Loilome W. Lipidomic Profiles of Cancer-Associated Fibroblasts with Cholangiocarcinoma Patients. SRIMEDJ [internet]. 2025 Feb. 26 [cited 2025 Mar. 30];40(1):93-104. available from: https://li01.tci-thaijo.org/index.php/SRIMEDJ/article/view/265779

Issue

Vol. 40 No. 1 (2025): January - February

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Original Articles

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