The Association of Multidrug Resistance Gene G2677T/A Genotypes and Cervical Cancer

Abstract

Background and objectives: The multidrug resistance 1 gene (MDR1) plays an important role in function of P-gp to excrete toxins from cells. A nucleotide at position 2677 of MDR1 is polymorphic with 6 genotypes:  GG GA GT TA TT and AA, an individual’s genotype might contribute to carcinogenesis. This study was to investigate the association between genetic polymorphism of MDR1 (G2677A/T) and risk for cervical cancer.

Methods: A case-control study conducted 204 patients with squamous cell carcinoma of cervix and 204 age-matched healthy controls (5-year interval). Genotypic MDR1 (G2677A/T) was detected from buffy coat by using real time polymerase chain reaction with TaqMan Probe. The association between MDR1 (G2677A/T) genotype and risk of cervical cancer was analyzed by using logistic regression.

Results: The association between genotype of MDR1 at position 2677A/T cervical cancer risk was not observed (p > 0.05). However, among positive-HPV women with TT genotype showed a trend to increased risk for cervical cancer OR= 3.10 (95%CI = 0.72-18.58, p = 0.0904) and adjusted OR =3.89 (95%CI = 0.95-15.90, p = 0.059) for smoking and contraceptive use.

Conclusions: Our data suggest that MDR1 (G2677A/T) polymorphism is not associated with risk of cervical cancer in Thai women. HPV infection with TT genotype may promote cervical cancer development. Therefore, investigation of genetic risk factor may be a useful method for screen women at high risk of cervical cancer.