Quality of Blood Components for Transfusion in Blood Transfusion Centre, Faculty of Medicine, Khon Kaen University, Thailand.

Abstract

Background and Objective : There are recommendations for the quality of blood components from the  Council of Europe (EU), American Association of  Blood Banks (AABB) and National Blood Centre, Thai Red Cross Society (TRC). We analyzed the quality control (QC) of blood components processed by the routine method used in our blood bank. This study aimed to test whether the components reached the recommended quality.

Methods : Packed red cells (PRC), leukocyte poored red cells (LPRC) and leukocyte depleted red cells(LDB) were measured hematocrit, weights and volumes were calculated based on specific gravity. Plasma were measured for volumes. Random platelet concentrates(RDP), leukocyte poored platelet concentrates(LPPC) were measured for volumes and platelet content. Cryoprecipitate were measured factor VIII and fibrinogen. For counting residual leukocytes, they were performed in LPRC, LDB, RDP and LPPC. Sterility test were done by sampling from all types of blood components.

Results : Red blood cell concentrates volume was 150-330 mL (refer to method used), with 50-80% of hematocrit. More than 90% of leukocyte poored red cells had fewer than 1.2X10⁹ white blood cells/unit and more than 90% of leukocyte depleted red cells(LDB) had fewer than 1X10⁶ white blood cells/unit. Plasma volume was ≥ 150 mL. Cryoprecipitate showed more than 80 IU/unit of factor VIII and more than 150 IU/unit for fibrinogen. Random platelet concentrates(RDP) had content more than 5.5X10¹⁰ cells/unit. Leukocyte poored platelet concentrates(LPPC) were more than 24X10¹⁰ cells/unit and had fewer than 1X10⁹ white blood cells contamination. Sterility test were no growth 100%.

Conclusions : Our QC data of blood  components provides reached the recommended quality of  EU, AABB, TRC.