Effect of Hesperidin on L-NAME-Induced Hypertension and Vascular Dysfunction in Rats
Keywords:
Hesperidin, L-NAME-induced hypertensive rats, Vascular dysfunction, เฮสเพอริดิน, หนูแรทที่ถูกชักนำให้เกิดความดันเลือดสูงด้วยสารแอลเนม, การทำงานของหลอดเลือดที่ผิดปกติAbstract
Background and Objectives: Hesperidin is a flavonoid found in peels or juices ofcitrus fruits. The beneficial effects of hesperidin have been reported such as anti-inflammation and antioxidation. This study was to investigate whether hesperidin could alleviate Nω-Nitro-l-arginine methyl ester(L-NAME)-induced hypertension in rats
Methods: Male Sprague-Dawley ratswere induced hypertension by L-NAME administration. Rats were treated with L-NAME (40 mg/kg/day) in drinking water only or together with hesperidin (15 and 30mg/kg BW per day) for five weeks while control rats received distilled water. Systolic blood pressure was measured weekly. Vascular function test was performed in aortic rings and mesenteric vascular beds.
Results: Rats treated with L-NAME had high blood pressure and a decrease in acetylcholine(ACh)-induced vasorelaxation in isolated aortic rings and mesenteric vascular beds. Vascular response to sodium nitroprusside(SNP) did not differ among groups. Interestingly, hesperidin significantly prevented L-NAME-induced hypertension and alleviated L-NAME-induced vascular dysfunction (p<0.05).
Conclusion: These findings suggested that hesperidin partially prevent the development of hypertension induced by L-NAME. This protective effect could involve the improvement of endothelium-dependent vasorelaxation in rats treated with L-NAME.
