IN VITRO BIOLOGICAL ACTIVITIES OF Lonicera japonica FLOWERING BUD COMPARED TO CHLOROGENIC ACID, ROSMARINIC ACID AND CAFFEIC ACID

Authors

  • CHAYANON CHAOWUTTIKUL College of Public Health Sciences, Chulalongkorn University, Bangkok
  • CHANIDA PALANUVEJ College of Public Health Sciences, Chulalongkorn University, Bangkok
  • NIJSIRI RUANGRUNGSI College of Public Health Sciences, Chulalongkorn University, Bangkok AND Faculty of Pharmacy, Rangsit University, Patumthani

DOI:

https://doi.org/10.69598/tbps.13.1.27-34

Keywords:

Lonicera japonica, flowering bud, chlorogenic acid, rosmarinic acid, caffeic acid, cytotoxicity, antimicrobial

Abstract

Lonicera japonica Thub. or Japanese honeysuckle has been widely used in traditional medicine to treat many ailments. Chlorogenic acid, rosmaric acid and caffeic acid are hydroxycinnamic acid derivatives found in this plant part. This study investigated in vitro cytotoxic and antimicrobial of L. japonica flowering bud as well as chlorogenic acid, rosmarinic acid and caffeic acid. Dried L. japonica flowering bud was exhaustively extracted with 95% ethanol. Cytotoxic activities were performed by brine shrimp lethality assay, MTT cell viability assay (5 cancer and 1 normal cell lines) and comet assay. Antibacterial activities against 11 bacterial strains were determined. L. japonica flowering bud ethanolic extract showed non-toxicity on brine shrimp nauplii and 6 tested cell lines. Chlorogenic acid, rosmarinic acid and caffeic acid demonstrated toxicity against brine shrimp nauplii. They showed more cytotoxic potentials against tested cell lines than the extract but were still accepted as no cytotoxicity. The extract and 3 compounds showed human lymphocyte DNA damage by comet assay. They showed no inhibitory activities against tested microorganisms.

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Published

2018-03-15

How to Cite

CHAOWUTTIKUL, C., PALANUVEJ, C., & RUANGRUNGSI, N. (2018). IN VITRO BIOLOGICAL ACTIVITIES OF Lonicera japonica FLOWERING BUD COMPARED TO CHLOROGENIC ACID, ROSMARINIC ACID AND CAFFEIC ACID. Thai Bulletin of Pharmaceutical Sciences, 13(1), 27–34. https://doi.org/10.69598/tbps.13.1.27-34

Issue

Vol. 13 No. 1 (2018): JANUARY - JUNE 2018

Section

Original Research Articles

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