AN APPLICATION OF TLC-IMAGE ANALYSIS FOR INVESTIGATION OF AQUEOUS EXTRACT OF COMMON CULINARY HERBAL COMBINATION, KAFFIR LIME LEAF, GALANGAL AND LEMON GRASS RHIZOMES

Authors

  • Panadda Phattanawasin Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Silpakorn University, Sanamchandra Palace Campus, Nakhon Pathom
  • Jankana Burana-osot Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Silpakorn University, Sanamchandra Palace Campus, Nakhon Pathom
  • Kanchana Kengkoom National Laboratory Animal Center, Mahidol University, Nakhon Pathom
  • Uthai Sotanaphun Department of Pharmacognosy, Faculty of Pharmacy, Silpakorn University, Sanamchandra Palace Campus, Nakhon Pathom

DOI:

https://doi.org/10.69598/tbps.14.1.27-33

Keywords:

TLC-image analysis, kaffir lime leaf, galangal rhizome, lemon grass rhizome, fingerprint

Abstract

Kaffir lime leaf, galangal, and lemon grass rhizomes are ones of the common kitchen herbs used as flavoring ingredients in famous traditional Thai cuisine, called TomYum. These herbs are also employed as herbal medicines for a wide range of applications. A number of studies were conducted to investigate phytochemical constituents of these household remedy, however, the gas chromatographic (GC) and high performance liquid chromatographic (HPLC) analyses of volatile oils or essential oils in each individual herbal extract prepared by low to partial polar organic solvents were mainly focused. Since combinations of these herbs are usually prepared by brewing with hot water, therefore, this study presented the use of TLC-image analysis method for visualization of all detectable constituents in the aqueous extract of the multi-ingredient preparation. Good chromatographic separations of various substance classes were achieved on silica gel 60 F254S TLC plates with the use of several mobile phase compositions, multiple detections and TLC-image analysis software, Sorbfil TLC Videodensitometer, to create a chromatographic profile.

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Published

2018-09-19

How to Cite

Phattanawasin, P., Burana-osot, J., Kengkoom, K., & Sotanaphun, U. (2018). AN APPLICATION OF TLC-IMAGE ANALYSIS FOR INVESTIGATION OF AQUEOUS EXTRACT OF COMMON CULINARY HERBAL COMBINATION, KAFFIR LIME LEAF, GALANGAL AND LEMON GRASS RHIZOMES. Thai Bulletin of Pharmaceutical Sciences, 14(1), 27–33. https://doi.org/10.69598/tbps.14.1.27-33

Issue

Vol. 14 No. 1 (2019): JANUARY - JUNE 2019

Section

Original Research Articles

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