DEVELOPMENT OF CREAM FORMULATION CONTAINING TETRAGONULA PAGDENI PROPOLIS FROM MANGOSTEEN ORCHARDS AND EVALUATION OF ANTIBACTERIAL ACTIVITY AGAINST STAPHYLOCOCCUS AUREUS
DOI:
https://doi.org/10.69598/tbps.14.2.93-103Keywords:
stingless bees, propolis, antibacterial, Staphylococcus aureus, creamAbstract
This study investigated antibacterial activity of the propolis extract of Thai stingless bee Tetragonula pagdeni (Schwarz) against Staphylococcus aureus and formulated a topical antibacterial cream. The stingless bee propolis was collected from mangosteen orchards. The propolis sample was extracted using ethanol as a solvent and partitioned with methanol and hexane. The extraction method provided a yield of 14.15% (w/w). The alpha-mangostin content in the propolis extract was 13.40±0.03% as determined by high performance liquid chromatography. Antibacterial susceptibility was determined by disc diffusion method. The propolis extract showed inhibition zone against S. aureus. The MIC and the MBC values of the propolis extract were examined by broth microdilution method and were found to be 3.06 and 6.12 µg/ml, respectively. The propolis cream formulation containing 5% propolis extract was developed. The propolis cream formulation was yellow with good texture and good spreadability with pseudoplastic and thixotropic properties. The 4-fold dilution of propolis cream formulation with culture media showed bactericidal activity against S. aureus. This study shows that propolis extract of Tetragonula pagdeni (Schwarz) is a promising candidate for the development of topical antibacterial dosage forms.
References
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2. Lu LC, Chen YW, Chou CC. Antibacterial activity of propolis against Staphylococcus aureus. Int J Food Microbiol. 2005;102:213-20.
3. Santana HF, Barbosa AA, Ferreira SO, Mantovani HC. Bactericidal activity of ethanolic extracts of propolis against Staphylococcus aureus isolated from mastitic cows. World J Microbiol Biotechnol. 2012;28:485-91.
4. Wagh VD. Propolis: a wonder bees product and its pharmacological potentials. Adv Pharmacol Sci. 2013;2013:308249.
5. Miguel MG, Antunes MD. Is propolis safe as an alternative medicine? J Pharm Bioallied Sci. 2011;3(4):479-95.
6. Wojtyczka RD, Kępa M, Idzik D, Kubina R, Kabała-Dzik A, Dziedzic A, et al. In vitro antimicrobial activity of ethanolic extract of Polish propolis against biofilm forming Staphylococcus epidermidis strains. Evid Based Complement Alternat Med. 2013;2013:590703.
7. Silici S, Kutluca S. Chemical composition and antibacterial activity of propolis collected by three different races of honeybees in the same region. J Ethnopharmacol. 2005;99:69-73.
8. Fokt H, Pereira A, Ferreira AM, Cunha A, Aguiar C. How do bees prevent hive infection? The antimicrobial properties of propolis. In: Mendez-Vilas A, editor. Current research, technology and education topics in applied microbiology and microbial biotechnology (Microbiology book series; 2). Badajoz (Spain): Formatex Research Center; 2010. p.481-93.
9. Umthong S, Puthong S, Chanchao C. Trigona laeviceps propolis from Thailand: antimicrobial, antiproliferative and cytotoxic activities. Am J Chin Med. 2009;37(5):855-65.
10. Schnitzler P, Neuner A, Nolkemper S, Zundel C, Nowack H, Sensch KH, et al. Antiviral activity and mode of action of propolis extracts and selected compounds. Phytother Res. 2010;24:S20-8.
11. Xuan H, Li Z, Yan H, Sang Q, Wang K, He Q, et al. Antitumor activity of Chinese propolis in human breast cancer MCF-7 and MDA-MB-231 cells. Evid based Complement Alternat Med. 2014;2014:280120.
12. Banskota AH, Tezuka T, Adyana IK, Midorikawa K, Matsushige K, Message D, et al. Cytotoxic, hepatoprotective and free radical scavenging effects of propolis from Brazil, Peru, the Netherlands and China. J Ethnopharmacol. 2000;72:239-46.
13. Park EH, Kim SH, Park SS. Anti-inflammatory activity of propolis. Arch Pharm Res. 1996;19(5):337-41.
14. Mayes DM, Bhatta CP, Shi D, Brown JC, Smith DR. Body size influences stingless bee (Hymenoptera: Apidae) communities across a range of deforestation levels in Rondônia, Brazil. J Insect Sci. 2019;19(2):23.
15. Kaškonienė V, Venskutonis PR. Floral markers in honey of various botanical and geographic origins: a review. Compr Rev Food Sci Food Saf. 2010;9:620-34.
16. Kongkiatpaiboon S, Vongsak B, Machana S, Weerakul T, Pattarapanich C. Simultaneous HPLC quantitative analysis of mangostin derivatives in Tetragonula pagdeni propolis extracts. J King Saud Univ Sci. 2016;28:131-5.
17. Huang S, Zhang C, Wang K, Li GQ, Hu F. Recent advances in the chemical composition of propolis. Molecules. 2014;19(12):19610-32.
18. Vongsak B, Kongkiatpaiboon S, Jaisamut S, Machana S, Pattarapanich C. In vitro alpha glucosidase inhibition and free-radical scavenging activity of propolis from Thai stingless bees in mangosteen orchard. Rev Bras Farmacogn. 2015;25:445-50.
19. Ahmad M, Yamin BM, Lazim AM. A study on dispersion and characterisation of α-mangostin loaded pH sensitive microgel systems. Chem Cent J. 2013;7:85.
20. Rasyid R, Fauziah F, Akbar R. Validation of analysis method α-mangostin compound on skin fruit extract kandis acid (Garcinia iowar Rob. Ex Choisy) method HPLC. Der Pharma Chemica. 2017;9(7):94-8.
21. Tong SYC, Davis JS, Eichenberger E, Holland TL, Fowler VG Jr. Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. Clin Microbiol Rev. 2015;28(3):603-61.
22. van Staden ADP, Heunis T, Smith C, Deane S, Dicks LMT. Efficacy of lantibiotic treatment of Staphylococcus aureus-induced skin infections, monitored by in vivo bioluminescent imaging. Antimicrob Agents Chemother. 2016;60(7):3948-55.
23. Malachowa N, Kobayashi SD, Braughton KR, DeLeo FR. Mouse model of Staphylococcus aureus skin infection. In: Allen IC, editor. Mouse models of innate immunity: methods and protocols (Methods in molecular biology; 1031). Totowa (NJ): Humana Press; 2013. p.109-16.
24. Mölne L, Tarkowski A. An experimental model of cutaneous infection induced by superantigen-producing Staphylococcus aureus. J Invest Dermatol. 2000;114(6):1120-5.
25. National Committee for Clinical Laboratory Standards. Method for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard M7-A6. 6th ed. Wayne (PA); The Committee; 2003.
26. Chen MX, Alexander KS, Baki G. Formulation and evaluation of antibacterial creams and gels containing metal ions for topical application. J Pharm. 2016;2016:5754349.
27. Deuschle VCKN, Deuschle RAN, Bortoluzzi MR, Athayde ML. Physical chemistry evaluation of stability, spreadability, in vitro antioxidant, and photo-protective capacities of topical formulations containing Calendula officinalis L. leaf extract. Braz J Pharm Sci. 2015;51(1):63-75.
28. Sureshjani MH, Yazdi FT, Mortazavi SA, Behbahani BA, Shahidi F. Antimicrobial effects of Kelussia odoratissima extracts against food borne and food spoilage bacteria "in vitro". J Paramed Sci. 2014;5(2):115-20.
29. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing: twenty-first informational supplement M100-S21. Wayne (PA); The Institutes; 2011.
30. Iinuma M, Tosa H, Tanaka T, Asai F, Kobayashl Y, Shimano R, et al. Antibacterial activity of xanthones from guttiferaeous plants against methicillin-resistant Staphylococcus aureus. J Pharm Pharmacol. 1996;48:861-5.
31. Chomnawang MT, Surassmo S, Wongsariya K, Bunyapraphatsara N. Antibacterial activity of Thai medicinal plants against methicillin-resistant Staphylococcus aureus. Fitoterapia. 2009;80:102-4.
32. French GL. Bactericidal agents in the treatment of MRSA infections--the potential role of daptomycin. J Antimicrob Chemother. 2006;58:1107-17.
33. Koh JJ, Qiu S, Zou H, Lakshminarayanan R, Li J, Zhou X, et al. Rapid bactericidal action of alpha-mangostin against MRSA as an outcome of membrane targeting. Biochim Biophys Acta. 2013;1828:834-44.
34. Phuong NTM, Van Quang N, Mai TT, Anh NV, Kuhakarn C, Reutrakul V, et al. Antibiofilm activity of α-mangostin extracted from Garcinia mangostana L. against Staphylococcus aureus. Asian Pac J Trop Med. 2017;10(12):1154-60.
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Published
2019-08-06
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Chuapaditphun, P., Kajhonlit, B., Panyakawin, S., & Wannachaiyasit, S. (2019). DEVELOPMENT OF CREAM FORMULATION CONTAINING TETRAGONULA PAGDENI PROPOLIS FROM MANGOSTEEN ORCHARDS AND EVALUATION OF ANTIBACTERIAL ACTIVITY AGAINST STAPHYLOCOCCUS AUREUS. Thai Bulletin of Pharmaceutical Sciences, 14(2), 93–103. https://doi.org/10.69598/tbps.14.2.93-103
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