FINGERPRINT STUDY AND QUANTIFICATION OF BIOACTIVE GENISTEIN GLYCOSIDE IN DERRIS SCANDENS STEM AND POLYHERBAL FORMULATIONS BY TLC-IMAGE ANALYSIS
DOI:
https://doi.org/10.69598/tbps.17.1.1-11Keywords:
TLC-image analysis, Derris scandens, polyherbal formulation, fingerprint, quantification, genistein-7-O-[-rhamnopyranosyl-(16)]--glucopyranosideAbstract
The simple and efficient thin-layer chromatographic (TLC)–image analysis method was applied to the fingerprint study and quantitative determination of an active ingredient, genistein-7-O-[a-rhamnopyranosyl-(1®6)]-b-glucopyranoside, in the dried powder of Derris scandens stem and its polyherbal formulations. The fingerprint analysis was performed on silica gel 60 F254 TLC plates with the use of various solvent systems and detections; whereas, the quantitation of the active compound was achieved by using the previously reported TLC-image analysis method processed with two types of analysis software, UN-SCAN-IT and ImageJ. Evaluating the similarity between different samples could be clearly acquired from the fingerprints constructed from stored TLC images. The profile plots of all images generated by using the TLC-image analysis software was also useful for quantifying the amount of the active component in the complex polyherbal formulations. The resulting amount obtained from the TLC‒image analysis method was found to be comparable to the ones obtained from the TLC-densitometric and HPLC methods, and the content of GTG found in different samples was significantly different and ranged from 0.01 to 2.2 %w/w.
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