FINGERPRINT STUDY AND QUANTIFICATION OF BIOACTIVE GENISTEIN GLYCOSIDE IN DERRIS SCANDENS STEM AND POLYHERBAL FORMULATIONS BY TLC-IMAGE ANALYSIS
DOI:
https://doi.org/10.14456/tbps.2022.1Keywords:
TLC-image analysis, Derris scandens, polyherbal formulation, fingerprint, quantification, genistein-7-O-[-rhamnopyranosyl-(16)]--glucopyranosideAbstract
The simple and efficient thin-layer chromatographic (TLC)–image analysis method was applied to the fingerprint study and quantitative determination of an active ingredient, genistein-7-O-[a-rhamnopyranosyl-(1®6)]-b-glucopyranoside, in the dried powder of Derris scandens stem and its polyherbal formulations. The fingerprint analysis was performed on silica gel 60 F254 TLC plates with the use of various solvent systems and detections; whereas, the quantitation of the active compound was achieved by using the previously reported TLC-image analysis method processed with two types of analysis software, UN-SCAN-IT and ImageJ. Evaluating the similarity between different samples could be clearly acquired from the fingerprints constructed from stored TLC images. The profile plots of all images generated by using the TLC-image analysis software was also useful for quantifying the amount of the active component in the complex polyherbal formulations. The resulting amount obtained from the TLC‒image analysis method was found to be comparable to the ones obtained from the TLC-densitometric and HPLC methods, and the content of GTG found in different samples was significantly different and ranged from 0.01 to 2.2 %w/w.
References
The National List of Essential Medicine [in Thai]. [homepage on the Internet]. C2012 [updated 2012 Aug 21; cited 2021 Apr 9]. Available from http://kpo.moph.go.th/webkpo/tool/ Thaimed2555.pdf
Puttarak P, Sawangjit R, Chaiyakunapruk N. Efficacy and safety of Derris scandens (Roxb.) Benth. for musculoskeletal pain treatment: A systematic and meta-analysis of randomized controlled trials. J Ethnopharmacol. 2016; 194:316–23.
Kuptniratsaikul V, Pinthong T, Bunjob M, Thanakhumtorn S, Chinswangwatanakul P, Thamlikitkul V. Efficacy and safety of Derris scandens Benth extracts in patients with knee osteoarthritis. J Altern Complement Med. 2011; 17:147–53.
Srimongkol Y, Warachit P, Chavalittumrong P, Sriwanthana B, Pairour R, Inthep C, Suphaphon B, Wongsinkongman P. A study of the efficacy of Derris scandens (Roxb.) Benth. extract compared with diclofenac for the alleviation of low back pain. J Thai Trad Alt Med. 2007; 5:17–23.
Benchakanta S, Puttiwong S, Boontan N, Wichit M, Wapee S, Kansombud S. A comparison of efficacy and side effects of knee osteoarthritis treatments with crude Derris scandens and ibuprofen. J Thai Trad Alt Med . 2012; 10:115–23.
Punjanon T. The Additivity antinociceptive interactions between diclofenac and the Derris scandens extract drug in mice. Asian J Pharm Clin Res. 2018; 11(1):314-17.
TTM Service. [homepage on the Internet]. [cited 2021 Apr 29]. Available from: http://hs.dtam.moph.go.th/
Yongyu Z, Shujun S, Jianye D, Wenyu W, Huijuan C, Jianbing W, Xiaojun G. Quality control method for herbal medicine - chemical fingerprint analysis. In: Shoyama Y, editor. Quality control of herbal medicines and related areas. Croatia: InTech, 2011; p.171-94.
Li S, Han Q, Qiao C, Song J, Cheng CL, Xu H. Chemical markers for the quality control of herbal medicines: an overview. Chinese Medicine. 2008; 3:7.
Laupattarakasem P, Houghton PJ, Hoult JR. Anti-inflammatory isoflavonoids from the stems of Derris scandens. Planta Med. 2004; 70:496–501.
Thai Herbal Pharmacopoeia. Kaewjawjom Printing & Publishing Suan Sunandha Rajabhat University, 2018; 522–532.
Sheikh ZA, Shakeel S, Gul S, Zahoor A, Khan SS, Zaidi FH, Usmanghani K. A novel HPTLC method for quantitative estimation of biomarkers in polyherbal formulation. Asian Pac J Trop Biomed. 2015; 5(11): 955-9.
Reich E, Schibli A. High-performance thin-layer chromatography for the analysis of medicinal plants. New York: Thieme; 2007.
Lianga Y, Xieb P, Chanc K. Quality control of herbal medicines. J Chromatogr B. 2004; 812:53–70.
Phattanawasin P, Sotanaphun U, Kumsum A, Piyapolrungroj N, Chansiri G. A developed thin-layer chromatography method for the determination of genistein-7-O-[α-rhamnopyranosyl-(1 → 6)]-β-glucopyranoside in Derris scandens. J Planar Chromat. 2021; 34(1):55-60.
Ketmongkhonsit P, Chaichantipyuth C, Palanuvej C, Thitikornpong W, Sukrong S. A validated TLC-image analysis method for detecting and quantifying bioactive phyllanthin in Phyllanthus amarus and commercial herbal drugs. Songklanakarin J Sci Technol. 2015; 37 (3), 319-326.
Casoni D, Sima IA, Sarbu C. Thin-layer chromatography—an image processing method for the determination of acidic catecholamine metabolites. J Sep Sci. 2014; 37:2675–2681.
Sereshti H, Poursorkh Z, Aliakbarzadeh, G, Zarre S, Ataolahi S. An image analysis of TLC patterns for quality control of saffron based on soil salinity effect: A strategy for data (pre)-processing. Food Chem. 2018, 239:831-839.
Wongsinkongman P, Boonruad T, Techadamrongsin Y, Bansiddhi J, Chavalittumrong P. Chemical quality of Derris scandens (Roxb.) Benth. extract in 50% ethanol. J Thai Trad Alt Med. 2005; 1:86–101.
ImageJ. [homepage on the Internet]. [cited 2021 Apr 29]. Available from: https://imagej.net/Welcome
ICH Harmonized Tripartite Guideline (2005) Validation of analytical procedures: Test and methodology, International Conference on Harmonization, Geneva.
Wongsinkongman P, Bansiddhi J, Sanluangsin S, Thongjin T, Sothanapun U. Bioactive compounds of Derris scandens (Roxb.) Benth. Extract. 2013;11:267-279.
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