DEVELOPMENT OF HYDROGEL CONTAINING BACTERIAL CELLULOSE AND PECTIN OR ALGINATE FOR WOUND DRESSING APPLICATIONS
DOI:
https://doi.org/10.69598/tbps.17.2.23-36Keywords:
Bacterial cellulose, hydrogel, cross-linking, alginate, pectinAbstract
This study aimed to develop hydrogel containing bacterial cellulose (BC) for pharmaceutical applications. 2 %w/v BC was mixed with alginate (A) or pectin (P) at different concentrations with weight ratios of 3:7, 4:6 and 5:5. After that, the blend was fabricated by casting onto a mold and cross-linking with a 3 %w/v of calcium chloride solution to form a hydrogel, followed by a freeze-drying process. The morphological characterization, fluid uptake ability, swelling degree, water retention property and integrity value were evaluated. Our results show that 3-part of 2 %w/v BC mixed with 7-part of A or P at the concentrations
0.5 (BC2A0.5-1) and 5 %w/v (BC2P5-1) were the optimized concentration. The morphological characterization of BC2P5-1 showed a smooth surface, unlike BC2A0.5-1, which offered a fluff surface. The thickness and diameter of BC2A0.5-1 were lower than BC2P5-1 due to structural shrunk when cross-linking with 3 %w/v CaCl2 solution, but the hardness of BC2A0.5-1 was higher than BC2P5-1, while scanning electron microscope images showed no significant difference. Fluid uptake ability, swelling degree, water retention property and integrity value of BC2A0.5-1 cannot be evaluated because its structure was segregated after being soaked in phosphate buffer saline for 24 h. However, the fluid uptake ability and swelling degree of BC2P5-1 trended to increase and reached the equilibrium point after 8 h, and the water retention property trended to decrease and was constant after 10 h. Integrity value of BC2P5-1 was 30.85±0.50 %. In conclusion, the BC2P5-1 showed better properties than
BC2A0.5-1. Thus BC2P5-1 can be further developed as a prototype wound dressing.
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