PORCINE PLACENTA EXTRACT-LOADED MATRIX POLYMERIC-BASED DISSOLVING MICRONEEDLES FOR IMPROVING SKIN HYDRATION

Authors

  • Kritsanaporn Tansathien Pharmaceutical Development of the Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom
  • Worranan Rangsimawong Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand https://orcid.org/0000-0002-0753-0720
  • Praneet Opanasopit Pharmaceutical Development of the Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom https://orcid.org/0000-0002-4878-2529
  • Pattaranut Eakwaropas Faculty of Integrative Medicine, Rajamangala University of Technology Thanyaburi, Pathum Thani 12130, Thailand

DOI:

https://doi.org/10.14456/tbps.2022.13

Keywords:

dissolving microneedle, porcine placenta, polyvinylpyrrolidone, polyvinyl alcohol, hyaluronic acid, skin hydration

Abstract

Porcine placenta extract (PPE) is a macromolecular substance, that affects the limitation of permeability through the skin. The aim of this study was to fabricate matrix polymeric-based dissolving microneedles (DMNs) loaded with PPE for improving skin conditions. DMNs were developed by a mold-based method using polyvinylpyrrolidone K90, polyvinyl alcohol, and hyaluronic acid at a weight ratio of 2:0.5:1. The DMNs properties such as the morphology, PPE loading efficiency and capacity, the mechanical strength, skin insertion, in vitro dissolution, in vitro skin permeation, and stability were analyzed. Then, the in vivo human study was also evaluated to reveal the safety and effectiveness of PPE-loaded DMNs. The results showed that 3% w/w of PPE-loaded DMNs had a suitable morphology, good mechanical properties, complete insertion, and rapid dissolution into the skin. These DMNs exhibited high skin permeability of entrapped macromolecular protein. In the stability test, the DMNs showed a total protein content of over 80% at storage for a month in all temperatures. For in vivo human study, no skin irritation was found. Transepidermal water loss values increased, referring to the micro-holes created by DMNs for delivering PPE. Additionally, the PPE-loaded DMNs significantly increased skin hydration. Therefore, the good physical properties of 3% w/w PPE-loaded DMNs play an important role to enhance the PPE penetration through the skin and provide effective skin hydration without skin irritation.

References

Pogozhykh O, Prokopyuk V, Figueiredo C, Pogozhykh D. Placenta and placental derivatives in regenerative therapies: experimental studies, history, and prospects. Stem Cells Int. 2018;2018:4837930.

Nagae M, Nagata M, Teramoto M, Yamakawa M, Matsuki T, Ohnuki K, et al. Effect of porcine placenta extract supplement on skin condition in healthy adult women: a randomized, double-blind placebo-controlled study. Nutrients. 2020;12(6):1671.

Heo JH, Heo Y, Lee HJ, Kim M, Shin HY. Topical anti-inflammatory and anti-oxidative effects of porcine placenta extracts on 2,4-dinitrochlorobenzene-induced contact dermatitis. BMC Complement Altern. Med. 2018;18(1):331.

Han NR, Kim HY, Kim NR, Lee WK, Jeong H, Kim HM, et al. Leucine and glycine dipeptides of porcine placenta ameliorate physical fatigue through enhancing dopaminergic systems. Mol Med Rep. 2018;17(3):4120-30.

Laosam P, Panpipat W, Yusakul G, Cheong L-Z, Chaijan M. Porcine placenta hydrolysate as an alternate functional food ingredient: in vitro antioxidant and antibacterial assessments. PLOS ONE. 2021;16(10):e0258445.

Peña-Juárez MC, Guadarrama-Escobar OR, Escobar-Chávez JJ. Transdermal delivery systems for biomolecules. J Pharm Innov. 2022;17(2):319–32.

Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P. Molecular biology of the cell. 4th ed. New York: Garland Science; 2002. Available from: https://www.ncbi.nlm.nih.gov/books/NBK26830/

Kirkby M, Hutton ARJ, Donnelly RF. Microneedle mediated transdermal delivery of protein, peptide and antibody based therapeutics: current status and future considerations. Pharm Res. 2020;37(6):117.

Halder J, Gupta S, Kumari R, Gupta GD, Rai VK. Microneedle array: applications, recent advances, and clinical pertinence in transdermal drug delivery. J Pharm Innov. 2021;16(3):558-65.

Ahmed Saeed Al-Japairai K, Mahmood S, Hamed Almurisi S, Reddy Venugopal J, Rebhi Hilles A, Azmana M, et al. Current trends in polymer microneedle for transdermal drug delivery. Int. J Pharm. 2020;587:119673.

Dabholkar N, Gorantla S, Waghule T, Rapalli VK, Kothuru A, Goel S, et al. Biodegradable microneedles fabricated with carbohydrates and proteins: revolutionary approach for transdermal drug delivery. Int. J Biol Macromol. 2021;170:602-21.

Dalvi M, Kharat P, Thakor P, Bhavana V, Singh SB, Mehra NK. Panorama of dissolving microneedles for transdermal drug delivery. Life Sci. 2021;284:119877.

Waghule T, Singhvi G, Dubey SK, Pandey MM, Gupta G, Singh M, et al. Microneedles: a smart approach and increasing potential for transdermal drug delivery system. Biomed Pharmacother. 2019;109:1249-58.

Nagarkar R, Singh M, Nguyen HX, Jonnalagadda S. A review of recent advances in microneedle technology for transdermal drug delivery. J Drug Deliv Sci Technol. 2020;59:101923.

Rogero SO, Malmonge SM, Lugão AB, Ikeda TI, Miyamaru L, Cruz ÁS. Biocompatibility study of polymeric biomaterials. Artif Organs. 2003;27(5):424-7.

Jiao Y, Liu Z, Ding S, Li L, Zhou C. Preparation of biodegradable crosslinking agents and application in PVP hydrogel. J Appl Polym Sci. 2006;101(3);1515-21.

Hiremath P, Nuguru K, Agrahari V. Chapter 8 - material attributes and their impact on wet granulation process performance. In: Narang AS, Badawy SIF, editors. Handbook of pharmaceutical wet granulation. Elsevier; 2019. p.263-315.

Chen BZ, Ashfaq M, Zhang XP, Zhang JN, Guo XD. In vitro and in vivo assessment of polymer microneedles for controlled transdermal drug delivery. J Drug Target.. 2018;26(8):720-9.

Saha I, Rai VK. Hyaluronic acid based microneedle array: Recent applications in drug delivery and cosmetology. Carbohydr Polym. 2021;267:118168.

Price RD, Berry MG, Navsaria HA. Hyaluronic acid: the scientific and clinical evidence J Plast Reconstr Aesthet Surg. 2007;60(10):1110-9.

Albadr AA, Tekko IA, Vora LK, Ali AA, Laverty G, Donnelly RF, et al. Rapidly dissolving microneedle patch of amphotericin B for intracorneal fungal infections. Drug Deliv Transl Res. 2022;12(4):931-43.

Lee IC, He J-S, Tsai M-T, Lin K-C. Fabrication of a novel partially dissolving polymer microneedle patch for transdermal drug delivery. J Mater Chem B. 2015;3(2):276-85.

Waterborg JH. The lowry method for protein quantitation. In: Walker JM, editor. The protein protocols handbook. Totowa, NJ: Humana Press; 2009. p. 7-10.

Alexander H, Brown S, Danby S, Flohr C. Research techniques made simple: transepidermal water loss measurement as a research tool. J Invest Dermatol. 2018;138(11):2295-300.e1.

Larrañeta E, Moore J, Vicente-Pérez EM, González-Vázquez P, Lutton R, Woolfson AD, et al. A proposed model membrane and test method for microneedle insertion studies. Int J Pharm. 2014;472(1):65-73.

Zhang L, Guo R, Wang S, Yang X, Ling G, Zhang P. Fabrication, evaluation and applications of dissolving microneedles. Int J Pharm. 2021;604:120749.

Kathuria H, Lim D, Cai J, Chung BG, Kang L. Microneedles with tunable dissolution rate. ACS Biomater. Sci Eng 2020;(69):5061-8.

Wang M, Hu L, Xu C. Recent advances in the design of polymeric microneedles for transdermal drug delivery and biosensing. Lab Chip. 2017;17(8):1373-87.

Zhuang J, Rao F, Wu D, Huang Y, Xu H, Gao W, et al. Study on the fabrication and characterization of tip-loaded dissolving microneedles for transdermal drug delivery. Eur J Pharm Biopharm. 2020;157:66-73.

Park Y, Park J, Chu GS, Kim KS, Sung JH, Kim B. Transdermal delivery of cosmetic ingredients using dissolving polymer microneedle arrays. Biotechnol Bioprocess Eng.2015;20(3): 543-9.

Zhao J, Wu Y, Chen J, Lu B, Xiong H, Tang Z, et al. In vivo monitoring of microneedle-based transdermal drug delivery of insulin. J Innov Opt Health Sci. 2018;11(05):1850032.

Lee Y, Park S, Kim SI, Lee K, Ryu W. Rapidly detachable microneedles using porous water-soluble layer for ocular drug delivery. Adv Mater Technol. 2020;5(5):1901145.

Prabhu A, Jose J, Kumar L, Salwa S, Vijay Kumar M, Nabavi SM. Transdermal delivery of curcumin-loaded solid lipid nanoparticles as microneedle patch: an in vitro and in vivo study. AAPS Pharm Sci Tech. 2022;23(1):49.

Jamaledin R, Di Natale C, Onesto V, Taraghdari ZB, Zare EN, Makvandi P, et al. Progress in microneedle-mediated protein delivery. J Clin Med. 2020;9(2):542.

Tansathien K, Suriyaamporn P, Ngawhirunpat T, Opanasopit P, Rangsimawong W. A novel approach for skin regeneration by a potent bioactive placental-loaded microneedle patch: comparative study of deer, goat, and porcine placentas. Pharmaceutics. 2022;14(6):1221.

Stamatas GN, Zmudzka BZ, Kollias N, Beer JZ. Non-invasive measurements of skin pigmentation in situ. Pigment Cell Res. 2004;17(6):618-26.

Khosrowpour Z, Ahmad Nasrollahi S, Ayatollahi A, Samadi A, Firooz A. Effects of four soaps on skin trans-epidermal water loss and erythema index. J Cosmet Dermatol. 2019;18(3):857-61.

Zhou C-P, Liu Y-L, Wang H-L, Zhang P-X, Zhang J-L. Transdermal delivery of insulin using microneedle rollers in vivo. Int J Pharm. 2010;392(1):127-33.

Banks SL, Pinninti RR, Gill HS, Paudel KS, Crooks PA, Brogden NK, et al. Transdermal delivery of naltrexol and skin permeability lifetime after microneedle treatment in hairless guinea pigs. J Pharm Sci. 2010;99(7):3072-80.

Aioi A, Muromoto R, Mogami S, Nishikawa M, Ogawa S, Matsuda T. Porcine placenta extract reduced wrinkle formation by potentiating epidermal hydration. J Cosmet Dermatol sci. appl. 2021;11:101-9.

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Published

2022-08-11

Issue

Vol. 17 No. 2 (2022): JULY-DECEMBER 2022

Section

Original Research Articles

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