OPTIMIZATION AND EVALUATION OF MICROEMULSIONS CONTAINING GRAPE SEED OIL
DOI:
https://doi.org/10.14456/tbps.2023.7Keywords:
grape seed oil, microemulsion, polysorbate 80, Cetiol®, HE, , antioxidant activityAbstract
Grape seed oil, obtained from grape seeds (Vitis Vinifera L.), is used in cosmetic products. It demonstrates antifungal, antioxidant, anti-inflammation, and antibacterial activities. Microemulsions are a type of drug delivery system that has thermodynamic stability. Grape seed oil-loaded microemulsions were prepared by mixing water, oil, surfactant, and co-surfactant in an appropriate ratio. The objectives of this study were to develop grape seed oil-loaded microemulsions and to investigate the effects of surfactants (polysorbate 80 and polysorbate 20) and co-surfactants (ethanol, isopropyl alcohol, Cetiol® HE, and glycerin) on the physicochemical properties of the microemulsions. The stability was studied for 30 days at room temperature and protected from light. In addition, the antioxidant activity of grape seed oil and microemulsion formulations was analyzed. From the pseudo-ternary phase diagram, polysorbate 80, and Cetiol® HE at a ratio of 1:2 showed the largest microemulsion area. The amount of the loaded-grape seed oil and surfactant mixtures were 5-15% and 75-85%, respectively. All formulations were transparent light-yellow emulsions showing good thermodynamic stability. Moreover, surface charges, conductivity, pH, and viscosity were desirable. After the 30 day-stability tests, all microemulsion formulations presented good thermodynamic stability with no apparent changes observed. The antioxidant activity increased as grape seed oil was added in greater quantities. Despite the fact that the size of the particle was greater than 300 nm and the PDI value was high, the findings indicated that a microemulsion system containing grape seed oil, polysorbate 80, and Cetiol® HE could be utilized with good antioxidant activity.
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