Co-location of quantitative trait loci for drought and salinity tolerance in rice

Authors

  • Vaiphot Kanjoo Center for Agricultural Biotechnology, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom 73140, Thailand Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok 10900, Thailand School of Agriculture and Natural Resources, University of Phayao, Phayao 56000, Thailand
  • Suwat Jearakongman Sakon Nakhon Rice Research Center, Sakon Nakhon 47000, Thailand
  • Kanchana Punyawaew Rice Gene Discovery Unit, National Center for Genetic Engineering and Biotechnology, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom 73140, Thailand
  • Jonaliza L. Siangliw Rice Gene Discovery Unit, National Center for Genetic Engineering and Biotechnology, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom 73140, Thailand
  • Meechai Siangliw Rice Gene Discovery Unit, National Center for Genetic Engineering and Biotechnology, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom 73140, Thailand
  • Apichart Vanavichit Rice Science Center, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom 73140, Thailand
  • Theerayut Toojinda Rice Gene Discovery Unit, National Center for Genetic Engineering and Biotechnology, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom 73140, Thailand

DOI:

https://doi.org/10.14456/tjg.2011.3

Keywords:

drought tolerance, salinity tolerance, QTL, rice, chromosome segment substitution lines

Abstract

Drought and salinity stresses are major constraints limiting growth and productivity of rice plants in rainfed lowland areas of the Northeast of Thailand where Thai jasmine rice cultivar Khao Dawk Mali 105 (KDML105) is planted as majority. In this study, chromosome segment substitution lines (CSSL) of the KDML105 that carry quantitative trait loci (QTL) associated with drought tolerance (DT) located on chromosomes 1, 3, 4, 8 and 9 were evaluated for salinity tolerance (ST) at seedling stage. Seven CSS lines exhibited higher ST than the recipient KDML105. Some of these CSS lines displayed higher grain yield than KDML105 when tested in rainfed lowland. Eight QTL for ST were identified on chromosomes 1, 3, 4, 7, 8, 9, 10 and 12. Of these, four are located in the same position of the DT-QTL previously reported. The co-locations of DT and ST QTL may indicate a common mechanism for tolerance. In addition, CSSLs showing good performances under both drought and salinity stresses can be used as a new source of breeding materials in Thai rainfed lowland areas.

References

Alam, R., Sazzadur Rahman, M., Seraj, Z.I., Thomson, M.J., Ismail, A.M., Tumimbang-Raiz, E. and Gregorio, G.B. 2011. Investigation of seedling-stage salinity tolerance QTLs using backcross lines derived from Oryza sativa L. Pokkali. Plant Breeding 130: 430–437.

Bian, J.M., Jiang, L., Liu, L.L., Wei, X.J., Xiao, Y.H., Zhang, L.J., Zhao, Z.G., Zhai, H.Q. and Wan, J.M. 2010. Construction of a new set of rice chromosome segment substitution lines and identification of grain weight and related traits QTLs. Breeding Sci 60: 305–313.

Caetano, A. 1997. Resolving DNA amplification products using polyacrylamide gel electrophoresis and silver staining. In: R.M. Micheli and R. Bova (eds.), Fingerprinting Methods Based on PCR. Springer-Verlag, Heidelberg.

Ebitani, T., Takeuchi, Y., Nonoue, Y., Yamamoto, T., Takeuchi, K. and Yano, M. 2005. Construction and evaluation of chromosome segment substitution lines carrying overlapping chromosome segments of indica rice cultivar ‘Kasalath’ in a genetic background of japonica elite cultivar ‘Koshihikari’. Breeding Sci 55: 65–73.

Fang, P. and Wu, P. 2001. QTL X N-level interaction for plant height in rice (Oryza sativa L.). Plant Soil 236: 237–242.

Fukai, S. and Cooper, M. 1995. Development of drought-resistant cultivars using physio-morphological traits in rice. Field Crops Res 40: 67–86.

Gong, J.M., He, P., Qian, Q., Shen, L.S., Zhu, L.H. and Chen, S.Y. 1999. Identification of salt-tolerance QTL in rice (Oryza sativa L.). Chin Sci Bull 44: 68–71.

Gregorio, G.B. and Senadhira, D. 1993. Genetic analysis of salinity tolerance in rice (Oryza sativa L.). Theor Appl Genet 86: 333–338.

Heu, M.H. and Koh, H.J. 1991. Newly bred salt tolerant lines in rice. Kor J Breed 23: 59–63.

IRRI. 2002. Standard evaluation system for rice (SES). 2ed., Los Banos, Philippines.

Ishimaru, K., Kobayashi, N., Ono, K.. Yano, M. and Ohsugi, R. 2001. Are contents of rubisco, soluble protein and nitrogen in flag leaves of rice controlled by the same genetics? J Exp Bot 52: 1827–1833.

Kamoshita, A., Babu, R.C., Manikabda Boopathi, N. and Fukai, S. 2008. Phenotypic and genotypic analysis of drought-resistance traits for development of rice cultivars adapted to rainfed environments. Field Crops Res 109: 1–23.

Kanjoo, V., Punyawaew, K., Siangliw J.L. and Toojinda, T. 2007. Development of single QTL near isogenic line of KDML 105 for dissection of drought tolerance. In: The 2nd International Conference on Rice for the Future. Queen Sirikit National Convention Center, Bangkok, Thailand.

Kim, D.M., Ju, H.G., Kwon, T.R., Oh, C.S. and Ahn, S.N. 2009. Mapping QTLs for salt tolerance in an introgression line population between Japonica cultivars in rice. J Crop Sci Biotech 12: 121–128.

Koyama, M.L., Levesley, A., Koebner, R.M.D., Flower, T.J. and Yeo, A.R. 2001. Quantitative trait loci for component physiological traits determining salt tolerance in rice. Plant Physiol 125: 406–422.

Kubo, T., Aida, Y., Nakamura, K., Tsunematsu, H., Doi, K. and Yoshimura, A. 2002. Reciprocal chromosome segment substitution series derived from japonica and indica cross of rice (Oryza sativa L.). Breeding Sci 52: 319–325.

Lanceras, J.C., Pantuwan, G., Jongdee, B. and Toojinda, T. 2004. Quantitative trait loci associated with drought tolerance at reproductive stage in rice. Plant Physiol 135: 384–399.

Lee, S.Y., Ahn, J.H., Cha, Y.S., Yun, D.W., Lee, M.C., Ko, J.C, Lee, K.S. and Eun, M.Y. 2007. Mapping QTLs related to salinity tolerance of rice at the young seedling stage. Plant Breeding 126: 43–46.

Lilley, J., Ludlow, M., McCouch, S. and O’Toole, J.C. 1996. Locating QTL for osmotic adjustment and dehydration tolerance in rice. J Exp Bot 47 1427–1436.

Peng, Z., Wang, M., Li, F., Lv, H., Li, C. and Xia, G. 2009. A proteomic study of the response to salinity and drought stress in an introgression strain of bread wheat. Mol Cell Proteomics 8: 2676–2686.

Rabbani, M.A., Maruyama, K., Abe, H., Khan, M.A., Katsura, K., Ito, Y., Yoshiwara, K., Seki, M., Shinozaki, K. and Yamaguchi-Shinozaki, K. 2003. Monitoring expression profiles of rice genes under cold, drought, and high-salinity stresses and abscisic acid application using cDNA microarray and RNA gel-blot analyses. Plant Physiol 133: 1755–1767.

Robin, S., Pathan, M.S., Courtois, B., Lafitte, R., Carandang, S., Lanceras, S., Amante, M., Nguyen, H.T. and Li, Z. 2003. Mapping osmotic adjustment in an advanced back-cross inbred population of rice. Theor Appl Genet 107: 1288–1296.

Sabouri, H., Rezai, A., Moumeni, A., Kavousi, A., Katouzi, M. and Sabouri, A. 2009. QTLs mapping of physiological traits related to salt tolerance in young rice seedlings. Biol Plantarum 53: 657–662.

Suralta, R.R., Inukai, Y., and Yamauchi, A. 2008. Utilizing chromosome segment substitution lines (CSSLs) for evaluation of root responses to transient mositure stresses in rice. Plant Prod Sci 11: 457–465.

Thomson, M.J., Ocampo, M., Egdane, J., Rahman, M.A.,

Sajise, A.G., Adorada, D.L., Tumimbang-Raiz, E., Blumwald, E., Seraj, Z.I., Singh, R.K., Gregorio, G.B. and Abdelbagi, M. 2010. Characterizing the saltol quantitative trait locus for salinity tolerance in rice. Rice 3: 148–160.

Toojinda, T., Siangliw, J.L., Punyawaew, K. and Kanjoo, V. 2011. Development of single QTL near isogenic lines (NILs) of KDML105 for drought tolerance. Research Rep. National Center for Genetic Engineering and Biotechnology, NSTDA. Pathum Tanee, Thailand.

Vanavichit, A. 2007. Molecular diversity and evaluation of aromatic rice in Thailand. International Training-Workshop. Nakhon Pathom, Thailand. pp. 131–134.

Yi, M., New, K.T., Vanavichit, A., Chai-arree, W. and Toojinda, T. 2009. Marker assisted backcross breeding to improve cooking quality traits in Myanmar rice cultivar Manawthukha. Field Crops Res 113: 178–186.

Zeng, H., Zhong, Y. and Luo, L. 2006. Drought tolerance genes in rice. Genomics 6: 338–341.

Zhang, J., Zheng, H.G., Aarti, A., Pantuwan, G., Nguyen, T.T., Tripathy, J.N., Sarial, A.K., Robin, S., Babu, R.C., Nguyen, B.D., Sarkarung, S., Blum, A. and Nguyen, H.T. 2001. Locating genomic regions associated with components of drought resistance in rice: comparative mapping within and across species. Theor Appl Genet 103: 19–29.

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Published

2012-04-12

Issue

Vol. 4 No. 2 (2011): July-December 2011

Section

Research Articles