เทคนิคโปรตีโอมิกสำหรับการวิเคราะห์วิถีชีวสังเคราะห์สารพิษในสบู่ดำ
DOI:
https://doi.org/10.14456/tjg.2013.117Keywords:
Jatropha curcas, phorbol esters, proteomicsAbstract
Phorbol esters (PEs) are main toxins found in several parts of Jatropha curcas. The amount of PEs in Jatropha curcas differ by species depending on the climate and soil in each area. PEs are known to promote tumor growth and are poisonous to humans and animals. Several utilizations of Jatropha oil and Jatropha kernel meal obtained after oil extraction are therefore restricted. In this work, protein expression in Jatropha curcas fruits was studied to understand PEs biosynthetic pathway and its related pathway. Proteomics is a technique used to analyse changes in quantity and quality of protein expression with high efficiency and accuracy. The use of proteomics approach to study changes of PEs biosynthetic proteins in fruits would be highly beneficial in Jatropha curcas breeding to reduce PEs in the future.
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