Genetic abnormality of sperm in smoker
DOI:
https://doi.org/10.14456/tjg.2008.9Keywords:
cigarette, chromosome aberration, fluorescence in situ hybridization (FISH)Abstract
There are many chemical compounds in cigarette. They can cause several diseases on smokers such as heart disease and lung cancer. Mutagen is also found in cigarette. In this experiment, sex chromosomes of 21 sperm’s donors from 18 to 26 years old were studied (seven non-smokers as the control group and fourteen smokers as the experiment group). X and Y chromosome were detected with DNA probes by Fluorescence in situ hybridization (FISH). The results showed the significant difference in total sex chromosomes abnormality between experiment group and control group (13.07±10.194 and 5.29±1.890, respectively) (P<0.05) (mean±SD). Our study indicates that smoking cigarette causes numerical of sex chromosome aberration in sperm.
References
สำนักงานกองทุนสนับสนุนการสร้างเสริมสุขภาพ 2549. สถิติการตายจากการสูบบุหรี่ (http://www.thaihealth.or.th/news)
Baumgartner, A., Schid, T.E., Schuetz, C.G. and Adler, I.D. 2001. Detection of aneuploidy in rodent and human sperm by multicolor FISH after chronic exposure to diazepam. Mutat Res 490: 11-19.
Martin, R.H., Ernst, S., Rademaker. A., Barclay, L., Ko, E. and Summers, N. 1999. Analysis of sperm chromosome complements before, during, and after chemotherapy. Cancer Genet Cytogen 108: 133-136.
Naccarati, A., Zanello, A., Landi, S., Consigli, R., and Migliore, L. 2003. Sperm-FISH analysis and human monitoring: a study on workers occupationally exposed to styrene. Mutat Res 537: 131-140.
Potts, R.J., Newbury, C.J., Smith, G., Notarianni, L.J. and Jefferies, T.M. 1999. Sperm chromatin damage associated with male smoking. Mutat Res 423: 103-111.
Robbins, W.A., Vine, M.F., Truong, K.Y. and Everson, R.B. 1997. Use of fluorescence in situ hybridization (FISH) to assess effects of smoking, caffeine, and alcohol on aneuploidy load in sperm of healthy men. Environ Mol Mutagen 30: 175-183.
Rogelio, R., Wendy, A.R., Guadalupe, O., Victor, B., Javier, M., John, R.F., Rosa, M.G., Garcia, H. and Marriano, E.C. 2001. Organophosphorous pesticide exposure increases the frequency of sperm sex null aneuploidy. Environ Health Perspect 109: 1237-1240.
Rubes, J., Lowe, X., Moore, D., Perreault, S., Slott, V., Evenson, D., Selevan, S.G., and Wyrobek, A.J. 1998. Smoking cigarette is associated with increased sperm disomy in teenage man. Fertil Sterily 70: 715-723.
Shi, Q., Ko, E., Barcley, L., Hoang, T., Rademarker, A. and Martin, R. 2001. Cigarette smoking and aneuploidy in human sperm. Mol Reprod Dev 59: 417-421.
Taszarek, H.G., Depa-Martynow, M., Derwich, K., Pawelczyk, L. and Jedrzejczak, P. 2005. The influence of cigarette smoking on sperm quality of male smokers and nonsmokers in infertile couples. Przegl Lek 62: 978-981.
Xia, Y., Bian, Q., Xu, L., Cheng, S., Song, L., Liu, J., Wu, W., Wang, S. and Wang, X. 2004. Genotoxic effects on human spermatozoa among pesticide factory workers exposed to fenvalerate. Toxicol 203: 49-60.
Viloria, T., Rubio, M.C., Rodrigo, L., Calderon, G., Mercader, A., Mateu, E., Meseguer, M., Remohi, J. and Pellicer, A. 2005. Smoking habits of parents and male: female ratio in spermatozoa and preimplantation embryos. Human Repro 20: 2517-2522.
