A genetic screen of chitosan-resistant mutant in Arabidopsis thaliana
DOI:
https://doi.org/10.14456/tjg.2014.8Keywords:
chitosan, mutantAbstract
Forward genetic approach is a powerful tool to elucidate plant response to elicitors. Chitosan, the biodegradable polymer deacetylated form of chitin, plays a role as an elicitor in many plants. To discover the chitosan response mechanism, we achieved a screening for the EMS-mutagenized Arabidopsis population with chitosan-resistant characters that has not been previously reported. At the seedling stage, high dosage of chitosan reduced plant growth both shoot and root systems. Mutant phenotype is characterized by the larger plant size and/or longer root length compared with wild-type plant on 80 mg/L chitosan supplemented plates. Mutants also showed leaf defect phenotypes such as leaf shape and leaf coloration. A number of the putative mutants were sterile, with no flowers or showed early senescence before completion of their life cycles. At least 5 chitosan-insensitive mutant lines showed the larger shoots or longer root length comparing to wild type in chitosan treated condition. The genetic inheritance analysis with Ler background crosses showed heterosis phenotype, interfering the growth-related trait of chitosan-resistant characters. To avoid the heterosis interference, the mutants were crossed with Col background. To identify the mutant population with a single recessive mutated gene, F2 progeny of each cross was evaluated. Only three mutant lines, 106A, 129A and 161A showed the potential to contain the single recessive mutated genes involving in chitosan response. However, the 129A line did not showed the consistent chitosan-insensitive phenotype after the repeated phenotyping of the M3 population. Therefore, only 106A and 161A will be used for further chitosan-responsive gene characterization as the next step.
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