ความหลากหลายทางพันธุกรรมของพลับพลึงธาร พันธุ์ไม้น้ำใกล้สูญพันธุ์ในประเทศไทย และพืชในวงศ์ Amaryllidaceae โดยอาศัยเครื่องหมายโมเลกุลจำเพาะกับยีน Genetic variation of the endangered species, water-onion (Crinum thaianum) in Thailand and plants in family Amar

Abstract

Genetic variation among 77 samples of the endangered species, water-onion (Crinum thaianum) in Thailand and five plants in family Amaryllidaceae, including C. asiaticum, Hymenocallis littoralis, C. amabile, C. nathans and Hymenocallis sp., was studied using PCR-RFLP markers specific to seven genes, NADH dehydrogenase subunit F (ndhF),  Acetyl-CoA carboxylase beta subunit (accD), NADH-plastiquinone oxidoreductase subunit 7 (ndhH), NADH-plastoquinone oxidoreductase subunit 1 (ndhA), Photosystem I P700 apoprotein A2 (psaB), 18S rDNA and 26S rDNA. All samples could be separated into two main groups and 4 subgroups. Group I consisted of two subgroups, one was all water-onion samples and the other was C. asiaticum and C. amabile. Group II also consisted of two subgroups, one was Hymenocallis sp. and the other was H. littoralis and C. nathans. The developed PCR-RFLP markers could not distinguish all studied water-onion samples. Other DNA markers with high polymorphism and multi-locus detection should be used to study genetic variation among water-onion samples. Partial sequences of seven genes of water-onion were analyzed in order to confirm the gene specificity and for the specific identification of Thailand water-onion. Five partial gene sequences (accD, ndhH, ndhA, psaB, 26S rDNA) of water-onion were compared to other plant sequences available in database. The result indicated that water-onion was most closely related to C. asiaticum. This first report on water-onion gene sequences will be useful for the development of suitable DNA markers to evaluate water-onion genetic information and for conservation and sustainable use of water-onion in the future.