Marker-assisted selection to improve submergence tolerance, blast resistance and strong fragrance in glutinous rice

Authors

  • Phatsalakone Manivong Agriculture Research Center, National Agriculture and Forestry Research Institute, Vientiane 7170, Lao PDR
  • Siriporn Korinsak Rice Gene Discovery Unit, National Center for Genetic Engineering and Biotechnology, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom 73140, Thailand
  • Siripar Korinsak Rice Gene Discovery Unit, National Center for Genetic Engineering and Biotechnology, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom 73140, Thailand
  • Jonaliza Lanceras Siangliw Rice Gene Discovery Unit, National Center for Genetic Engineering and Biotechnology, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom 73140, Thailand
  • Apichart Vanavichit Rice Gene Discovery Unit, National Center for Genetic Engineering and Biotechnology, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom 73140, Thailand and Rice Science Center, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom 73140, Thailand
  • Theerayut Toojinda Rice Gene Discovery Unit, National Center for Genetic Engineering and Biotechnology, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom 73140, Thailand

DOI:

https://doi.org/10.14456/tjg.2014.13

Keywords:

marker-assisted, submergence, blast resistance, fragrance, glutinous rice

Abstract

     Glutinous rice is popularly grown for consumption in Lao PDR. Most of the Laotian glutinous rice varieties grew nowadays are intolerant to flash flooding, susceptible to major diseases such as blast and bacterial blight and lacking of good grain and cooking quality. Our goal of this study is to develop new glutinous rice varieties that have good cooking quality, tolerance to flash flooding and resistance to blast disease by combining Sub1, badh2, qBl1 and qBl11 loci from the glutinous elite lines IR85264 (Sub1), TDK303 (badh2) and RGD07529 (qBl1+qBl11) through marker-assisted foreground selection (MAS) strategy. Three-way crossing was made to develop a breeding population in which MAS was applied in the series to select for individuals carrying submergence tolerance (Sub1), blast resistance (qBl1, qBl11) and fragrance (badh2). According to the marker genotypes, twenty eight F5 homozygous lines carrying Sub1, badh2, qBl1 and qBl11 loci were finally selected and evaluated for submergence tolerance (SUBT), blast resistance (BLR) and fragrance (FR). All F5 selected lines are fragrance and showed high level of SUBT and BLR. This study provides further support on the effective of MAS to improve FR, SUBT and BLR in rice breeding program.

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Published

2014-08-31

Issue

Vol. 7 No. 2 (2014): MAY - AUG 2014

Section

Research Articles