Screening of lower fungal for unsaturated fatty acid production from leaves
DOI:
https://doi.org/10.14456/tjg.2010.15Keywords:
lower fungal, unsaturated fatty acid, ribosomal RNA (rRNA)Abstract
Ten isolates of lower fungi that were able to produce C14-C20 fatty acids were isolated from leaves of 26 natural sources in Thailand. Four Isolates that could synthesize high long-chain essential unsaturated fatty acids were selected. Isolates PB2001 and PB2002 produced g-linolenic acid 10.70% and 11.28%, respectively; isolate FAO001 produced arachidonic acid 12.48% and isolate PLL31 produced arachidonic and eicosapentaenoic acids 4.64% and 4.24%, respectively. Morphological studies of isolates PB2001 and PB2002 found globular shape of sporangium on top of sporangiophore, which could be expected for the genus Mucor. Isolates FAO001and PLL31 showed white and flat colony and flower-like growth, which could be expected for the genus Pythium. DNA extraction and PCR amplification of 18S rRNA (SSU) and 28S rRNA (LSU) genes and ITS region of the 4 isolates reviewed partial sequences of ITS, SSU and LSU. The comparison of ITS and LSU sequences to the GenBank database indicated that isolates PB2001 and PB2002 were similar to Mucor sp. up to 99%. The ITS, SSU, and LSU sequences of isolate FAO001 were similar to Pythium sp. at 93%, 91%, and 93%, respectively. The analysis for ITS and SSU sequences of isolate PLL31 were similar to Pythium sp. 97% and 95%, respectively.
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