Development of the Single Nucleotide Polymorphism markers for Phytophthora capsici resistance in Capsicum annuum
DOI:
https://doi.org/10.14456/tjg.2014.23Keywords:
DNA markers, resistant geneAbstract
Phytophthora capsici Leonian is one of the most important pathogens, which destruct the pepper crop (Capcicum sp.) in worldwide. The inheritance of Phytophthora resistant gene is complex inheritance, known as quantitative trait loci (QTL). The major QTL is located on chromosome 5. In this study, bulked segregant analysis (BSA) approach was used for discovery of polymorphic SNP markers in double haploid population derived from highly resistant Capsicum annuum accession Criollo de Morelos – 334 and susceptible variety Jindu. The linkage maps were constructed and QTL analysis was performed. The QTL with the highest LOD score, detected on chromosome 5, explained 31% of the phenotypic variance with a LOD score of 11. The Second QTL, designated as minor QTL, which was found on chromosome 8, explained 11.9% of the phenotypic with LOD score of 3.36. The nearest marker, which was located on major QTL and minor QTL were SNP_421 and SNP_694, respectively. Therefore, two SNP markers were the best candidate markers that linked to QTL of Phytophthora resistant gene.
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