Potential Role of Peptide Transporter2 Gene in Salt Stress
Keywords:
'Luang Pratahn', mutant, salt stress, peptide transporter, PTRAbstract
The Thai rice cultivar 'Luang Pratahn' is a promising candidate for a salt-tolerant donor plant. Previously, several key genes responsible for salt tolerance were identified using time-course transcriptomic data and weighted co-expression networks. The rice peptide transporter2 (OsPTR2) gene is one of the key genes with no prior information related to the salt tolerance mechanism in rice. Salt stress could induce OsPTR2 gene expression in salt tolerant cultivars, 'Pokkali' and 'Luang Pratahn', while it couldn’t be induced in IR29, the salt susceptible line, suggesting the possibility of OsPTR2 involvement in salt tolerance. Therefore, this research aims to investigate the role of PTR2 gene in salt stress response by using the OsPTR2 orthologous gene in Arabidopsis, AtPTR2. Based on bioinformatic analysis, OsPTR2 and AtPTR2 are transmembrane proteins and share 5 similar domains. Therefore, the homozygous line of Atptr2 knocked-out mutant line was used to investigate the involvement of Atptr2 gene in salt stress response. After 7 days under salt stress condition, Atptr2 mutant showed the significantly shorter root length and lower dry weight than wild type (WT), without significant difference in photosynthetic pigment contents between the mutant and WT. These results suggested that Atptr2 had a potential role in root development under salt stress condition. The revertant of Atptr2 mutant by OsPTR2 expression will be done for further characterization.
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