Low genetic variation of Boesenbergia tenuispicata, a species endangered and endemic to Thailand, using RAPD markers
DOI:
https://doi.org/10.14456/tjg.2012.16Keywords:
Boesenbergia tenuispicata, RAPD markers, genetic variationAbstract
Random amplified polymorphic DNA (RAPD) marker was used to analyze genetic structure of endemic Thai plant Boesenbergia tenuispicata K. Larsen that was sampled from its endemic regions about 20 samples of each population during January 2011 – January 2012 in Krabi, Thailand. Genomic DNA was extracted from fresh leaf of the samples. Nineteen RAPD primers were initially screened for analysis and eight primers (OPA-18, OPAM-01, OPAM-03, OPAM-12, OPB-14, OPD-03, OPD-18 and OPZ-03) were chosen for further analysis. Low polymorphic bands were detected by RAPD markers, indicating the genetic diversity of B. tenuispicata was low, and all populations most likely consisted of the same genotype. A total of 72 DNA fragments, varying from 100-2,800 bp, were amplified, of which 12 (16.66%) were polymorphic. A dendrogram was constructed based on polymorphic bands using the SPSS program (version 18). Three clusters could be separated with similarity coefficients ranging from 0.840-1.000. Therefore, RAPD is rapid and suitable method for analysis of genetic diversity among endemic B. tenuispicata samples. The result suggested that the management for the conservation of genetic variability in B. tenuispicata should aim to preserve every population.
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