Molecular Cytogenetic Aberrations in Thai Patients with Multiple Myeloma
Keywords:
multiple myeloma, Fluorescence, FISH, molecular cytogenetic, bone marrowAbstract
Cytogenetic abnormality plays an important role in prognosis of patients with multiple myeloma (MM). Conventional cytogenetic assay reveals limited results. In this study, we performed interphase fluorescence in situ hybridization (I-FISH) analysis to explore the incidence of cytogenetic abnormalities in patients with MM. Heparinized bone marrow was evaluated by FISH assay. Four FISH probes specific for deletion 13, deletion 17, t(4;14), and t(11;14) were used. Sixty-six patients were included from March 2013 to February 2014. The incidence of the abnormality was found in 35 of 66 cases (53.03%). Deletions of 13q14 region (D13S319) were detected in 19 cases (54.29%). Translocations involving 14q32 region were observed in 10 cases (28.57%) including t(4;14) and t(11;14) for 6 and 4 cases, respectively. Deletions of 17p13 region (TP53) were detected in 5 cases (14.29%). Furthermore, we found other abnormalities, such as, 3-4 copies of 11q13 (28.57%), 14q32 deletion (8.57%) and trisomy 17 (2.86%). It was unable to evaluate the results in 8/66 patients (12.1%). Clinical outcome and prognostic values are being followed. The most common cytogenetic abnormality finding in this study is 13q14.3 deletion. The prognostic impact on response to chemotherapy and survival among these patients needs to be determined in long-term follow-up.
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