Triamcinolone acetonide alters expressions of matrix metalloproteinase-3 gene in primary human chondrocytes
Keywords:
chondrocyte, osteoarthritis, glucocorticoid, triamcinolone acetonide, matrix metalloproteinaseAbstract
Intra-articular glucocorticoid (GC) injection such as triamcinolone acetonide is an ultimate pharmaceutical treatment for knee osteoarthritis (OA) patients who do not respond well to other pharmaceutical treatments. Effects of GCs on homeostasis of cartilage’s extracellular matrix (ECM) are not conclusive. In this study, we investigated whether TA caused destruction to ECM by altering expression of genes involved in digestion of collagens and proteoglycans. Chondrocytes isolated from cartilages of 10 knee OA patients were treated with TA at 1 and 5 mg/ml. Reverse-transcription real-time polymerase chain reaction was used for evaluating expressions of genes involving ECM degradation (matrix metalloproteinase (MMP)-1, MMP-3, MMP-13, ADAM with thrombospondin motives (ADAMTS)-5 and tissue inhibitor of matrix metalloproteinase (TIMP)-3). Statistical differences in relative-quantification expression levels between TA-treated chondrocytes and non-treated controls were found in MMP-3 (6.59 ± 5.2-fold for 1 mg/ml TA (p = 0.022) and 5.43 ± 3.46-fold for 5 mg/ml TA (p < 0.01)). Levels of MMP-1, MMP-13, ADAMTS-5, and TIMP-3 were not significantly changed. TA-increased expressions of MMP-3 in chondrocytes might assist further degradation of cartilage and accelerate OA progression. Results from this study may assist physicians in TA treatment planning for OA patients.References
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