Genetic relationship assessment of pineapple germplasm in Thailand revealed by AFLP markers
Keywords:
AFLPs, Ananas conosus, genetic relationship, pineappleAbstract
The study is aimed to depict phylogenetic relationship among 37 pineapple accessions collected from government research station, private company germplasm repository and commercial growing areas in Thailand using AFLP technique. Twenty–one primer combinations were used and 675 AFLP markers were produced and 468 markers (69.33%) were found to be polymorphic. Jaccard similarity coefficient among the samples was 0.62-1.0. Cluster analysis was performed using UPGMA method. The phylogenetic tree could separate all pineapple accessions into 9 groups at genetic similarity of 0.83. The first cluster, consisting of 14 accessions, which represented morphological characteristics of Cayenne group. The second cluster, consisting of 9 accessions, were in Queen group. The third cluster was White jewel, in Perolera group. Clusters 4-9 were hybrid, Spanish, Pernambuco groups and exotic varieties. Principle component analysis (PCA) was also performed and the results were in agreement with phylogenetic analysis. PCA1, 2 and 3 were count for 60.19% of total variation indicated moderate genetic diversity of the samples. The present studied also showed that AFLP technique clearly identified 4 commercial hybrid varieties close to Queen or Spanish group. This data will be useful for hybrid selection in breeding program and germplasm evaluation in the future.
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