Heat shock protein 70 gene polymorphism in KU–Phupan black–bone chicken
DOI:
https://doi.org/10.14456/gag.2017.2Keywords:
heat shock protein 70 gene, heat stress, KU–Phuphan black–bone chicken, PCR–RFLPAbstract
The objective of this study was to investigate the polymorphism of heat shock protein 70 (hsp70) gene in the F2 populations of KU–Phuphan black-bone chicken comparing with crossbreed native chicken and commercial layer chicken populations. Thirty female and twenty-five male KU–Phuphan black-bone chickens, and twenty-three for each crossbreed native chicken and commercial layer chicken were genotyped hsp70 gene by PCR–RFLP technique. The hsp70 gene showed two loci, locus C generated by EaeI and locus M created by MmeI. The locus C consisted of two alleles C1 and C2 providing three genotypes of C1C1, C1C2 and C2C2. The allele and genotype frequencies of KU–Phuphan black-bone chicken populations were not different from other Thai indigenous breeds. The locus M consisted of two alleles M1 and M2 whereas the two genotypes of M1M2 and M2M2 were observed. The allele M1 was low allele frequency and was only found in MB population whereas allele M2 showed monomorphism for FB, BR and LA populations. The male KU–Phuphan black-bone chicken exhibited different M1M2 genotype from other detected populations but the allele frequency of MB was less than other Thai indigenous chickens. The genotype frequency of candidate thermotolerance genotype C1C2/ M2M2 of both KU–Phuphan black-bone chicken populations was higher than other genotypes. According to the results, KU–Phuphan black-bone chickens showed variation genotypes especially for locus C and contained high genotype frequency of the candidate thermotolerance genotype. Therefore, the locus C and the candidate genotype of hsp70 gene could be used as genetic marker related with heat tolerance for selection and breeding program of KU–Phuphan black-bone chicken to create heat tolerance chickens.
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