The polymorphism analysis of gene resistance to avian influenza virus (Mx gene) in KU-Phuphan black-bone chicken
DOI:
https://doi.org/10.14456/gag.2017.9Keywords:
antiviral gene, Mx gene, PCR–RFLP, SNPAbstract
The Mx gene is an antiviral gene used to determine the resistance or the susceptibility to different types of viruses, including the Avian Influenza virus. Four populations of male (MB) and female (FB) KU–Phuphan black–bone chickens, crossbreed native broiler (BR) and commercial layer (LA) chicken were examined resistant and sensitive genotypes of Mx gene using PCR–RFLP. DNA amplification results showed that the Mx gene was found in a 100 bp fragment. The number of chicken showed homozygous resistant was 1, 4 and 6 for MB, FB and BR populations, respectively but could not found in LA population. The frequency of allele A was 42.6%, 25.6%, 54.3% and 45% for MB, FB, BR and LA populations, respectively. The frequency of allele G was 57.4%, 74.4%, 45.7% and 55.0%, for MB, FB, BR and LA populations, respectively. This implied the roughly equal frequencies between A and G allele of MB, BR and LA except FB populations. The sequence analysis of homozygous resistant and sensitive genotype confirmed the single nucleotide substitution (SNP) causes a single amino acid substitution from Serine (Ser) to Asparagine (Asn) at position 631, which correlated antiviral performance. Therefore, the SNP of Mx gene has been important for the poultry industry to protect their chickens from pathogenic infection.
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