Expression Analysis of Rice Polygalacturonase cDNA Responding to Brown Planthopper [Nilaparvata lugens (Stål)]

Sugunya Suebsan; Supranee Sitthiphrom; Kanta Sangwijit; Mondhon Sanguansermsri; Somboon Anuntalabhochai

doi:10.14456/gag.2017.3

Authors

Sugunya Suebsan University of Phayao
Supranee Sitthiphrom Loei Rajabhat University
Kanta Sangwijit University of Phayao
Mondhon Sanguansermsri University of Phayao
Somboon Anuntalabhochai University of Phayao

DOI:

https://doi.org/10.14456/gag.2017.3

Keywords:

polygalacturonase, Oryza sativa cv. KDML105, insect herbivore, ethylene, cytokinin

Abstract

A cDNA (OsKPG) encoding polygalacturonase (PG) from rice (Oryza sativa cv. KDML105) was cloned and sequenced. The cDNA full length was 978 bp and carried 277 deduced amino acids with 4 highly conserved domains among PG family. Consequently, the expression of OsKPG was investigated under brown planthopper [Nilaparvata lugens (Stål)] attack. The transcription of OsKPG under different phytohormone applications including ethylene, abscisic acid (ABA), 6-benzyladenine (6-BA) and 2,4-dichlorophenoxyacetic acid (2,4-D) were also analyzed in leaves. An RT-PCR revealed that the expression of OsKPG was high when faced with brown planthopper attack, application of ethylene or 6-BA treatments. This is the first evidence demonstrating that OsKPG may play a role to respond against insect herbivores through the signaling pathways between ethylene and cytokinin in rice.

Author Biographies

Sugunya Suebsan, University of Phayao

Biology Department, School of Science,

Supranee Sitthiphrom, Loei Rajabhat University

Faculty of Science and Technology

Kanta Sangwijit, University of Phayao

Biotechnology Unit

Mondhon Sanguansermsri, University of Phayao

Pharmaceutical Sciences Department, School of Pharmaceutical Sciences

Somboon Anuntalabhochai, University of Phayao

Biology Department, School of Science and Biotechnology Unit

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