Expression analysis and nucleotide variation of OsC1 gene associated with anthocyanin pigmentation in rice
DOI:
https://doi.org/10.14456/gag.2017.7Keywords:
OsC1 gen, Rice, Anthocyanins, Transcription factorAbstract
OsC1 gene encodes a R2R3-Myb transcription factor that regulates expression of structural genes in anthocyanins biosynthesis pathway in rice. Thus, the OsC1gene may play an important role for the coloration of rice pericarp. OsC1 gene expression in both leaf and seed tissues at different ages was examined in white, red and black rice. Expression of OsC1 gene was found in young leaf and seed tissues in all rice varieties in this study. In addition, black rice showed the highest expression level in seeds at 25 days after flowering, corresponded to dark purple (black) pericarp color. Comparisons of nucleotide sequences in exon 3 of OsC1 gene revealed 10-bp deletion in white and red rice, resulting in frameshift mutation in R3 region of Myb DNA binding domain. The result suggested that the OsC1 alleles of white and red rice were a loss-of-function mutation. The result from this study might be applied to rice improvement by developing molecular markers for selection of rice anthocyanin pigmentation.
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