Screening Thai landrace rice for blast resistance gene Pi9, Pi36, Pigm(t) using DNA markers

Authors

  • Kritkittisak Phaitreejit Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
  • Eng-orn Srikaew Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
  • Chatchawan Jantasuriyarat Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
  • Tanee Sriwongchai Department of Agronomy, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand
  • Sureeporn Kate-ngam Department of Agronomy, Faculty of Agriculture, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand

DOI:

https://doi.org/10.14456/tjg.2011.8

Keywords:

rice blast disease, DNA marker, resistant gene, landrace rice

Abstract

Rice blast caused by the fungal pathogenMagnaporthe grisea, is one of the most devastatingdiseases in rice production worldwide. Informationon rice blast resistant gene is important for ricecultivar development. The objective of this studywas to identify rice blast disease resistant genes in203 landrace Thai rice cultivars including 40 cultivars from the South, 19 cultivars from the North,144 cultivars from the Northeast of Thailandcomprised of 45 floating rice cultivars and 99lowland rice cultivars using gene specific markersfor blast resistant gene Pi9, Pi36 and Pigm(t). Theresults showed that all 203 cultivars have at leastone blast resistant gene and 42 cultivars have all thethree blast resistant genes. The outcome of thisresearch will be very useful for development of newblast resistance elite rice cultivars in the future.

References

ชลาวา บุรณศิริ. 2531. โรคพืชที่เกิดจากเชื้อรา.

สถาบันเทคโนโลยีพระจอมเกล้าเจ้าคุณทหาร

ลาดกระบัง, กรุงเทพฯ.

ชัชวาล จันทราสุริยารัตน์ และ สุรีพร เกตุงาม. 2552.

โรคไหม้ในข้าวและสถานการณ์ปัจจุบันของ

งานวิจัยด้านยีนต้านทานโรคไหม้ในข้าว.

วารสารแก่นเกษตร 37: 69-78.

ทัศนีย์ สงวนสัจ. 2540. บทบาทของพันธุกรรม

ต้านทานโรคและแมลงกับการปรับปรุงพันธุ์

ข้าวของไทย. ศูนย์วิจัยข้าวพิษณุโลก สถาบัน

วิจัยข้าว กรมวิชาการเกษตร กระทรวงเกษตร

และสหกรณ์.

พูนศักดิ์ เมฆวัฒนากาญจน์ พยอม โคเบลลี่ อัจฉาพร

ณ ลำปาง เนินพลับ ถนอมจิตร์ ฤทธิ์มนตรี

กุลชนา เกศสุวรรณ ชนสิริน กลิ่นมณี และ

สงวน เที่ยงดีฤทธิ์. 2550. การตรวจสอบความ

หลากหลายของสายพันธุ์เชื้อราสาเหตุโรคไหม้

ข้าวในประเทศไทย. Thai Rice Res J 1: 52-64.

Boman, J.M., Khush, G.S. and Nelson, R. 1992.

Breeding rice for resistance to pests. Annu Rev

Phytopathol 30: 507-528.

Bryan, G.T., Wu, K.S., Farrall, L., Jia, Y., Hershey,

H.P., McAdams, S.A., Faulk, K.N.,

Domaldson G.K., Tarchini, R. and Valent, B.

A single amino acid difference

distinguishes resistant and susceptible alleles

of the rice blast resistance gene Pi-ta. Plant

Cell 12: 2033-2046.

Chen, X., Shang, J., Chen, D., Lei, C., Zou, Y.,

Zhai, W., Lin, G., Xu, J., Ling, Z., Cao, G.,

Ma, B., Wang, Y., Zhao, X., Li, S. and Zhu, L.

A lectin receptor kinase gene conferring riceblast resistance. Plant J 46: 794

-804.

Deng, Y., Zhu, X., Shen, Y. and He, Z. 2006.

Genetic characterization and fine mapping of

the blast resistance locus Pigm(t) tightly

linked to Pi2 and Pi9 in a broad-spectrum

resistant Chinese variety. Theor Appl Genet

: 705-713.

Fukuoka, S. and Okuno, K. 2001. QTL analysis and

mapping of pi21, a recessive gene for field

resistance to rice blast in Japanese upland rice.

Theor Appl Genet. 103: 185-190.

Lin, F., Chen, S., Que, Z., Wang, L., Liu, X.Q. and

Pan, Q.H. 2007. The blast resistance gene Pi

encodes an NBS-LRR protein and is a

member of a resistance gene cluster on rice

chromosome 1. Genetics 177: 1871-1880.

Liu, G., Lu, G., Zen, L. and Wang, G.L. 2002. Two

broad-spectrum blast resistance genes, Pi9(t)

and Pi2(t), are physically linked on rice

chromosome 6. Mol Genet Genomics 267: 472

-480.

Liu, X., Lin, F., Wang, L. and Pan, Q. 2007. The in

silico map-based cloning of Pi36, a rice

coiled-coil-nucleotide-binding site-leucinerich

repeat gene that confers race-specific

resistance to the blast fungus. Genetics 176:

-2549.

Lodhi, M.A., Ye, G.N., Weeden, N.F. and Reisch,

B.I. 1994. Modification of a CTAB DNA

extraction protocol for plants containing high

polysaccharide and polyphenol components.

Plant Mol Biol Rept 12: 6-13.

Peng, S.Q., Huang, F.Y., Sun, G.C., Liu, E.M., Sun,

Y.J., Ai, R.X., Zhao, J.X., Bai, S.Z., Xiao,

F.H. 1996. Studies on durable resistance to blast disease in diVerent latitude for rice.

Scientia Agr Sinica 29: 52–58.

Qu, S., Liu, G., Zhou, B., Bellizzi, M., Zeng, L.,

Dai, L., Han, B. and Wang, G.L. 2006. The

broad spectrum blast resistance gene Pi9

encodes a nucleotide binding site-leucine-rich

repeat protein and is a member of a multigene

family in rice. Genetics 172: 1901-1914.

Rice knowledge bank. 2009. Introduction to rice

blast. Fungal diseases of rice. http://www.

knowledgebank.irri.org/ipm/index.php/

rice-blast (20 September 2009).

Sharma, T.M., Madhav, B., Singh, P., Shanker, T.,

Jana, V., Dalal, A., Pandit, A., Singh, K.,

Gaik, H. and Singh, N. 2005. High-resolution

mapping, cloning and molecular

characterization of the Pi-kh gene of rice,

which confer resistance to Magnaporthe

grisea. Mol Genet Genomics 274: 569-578.

Shen, Y., Adreit, H., Zhu, X.D., Milazzo, J.,

Chen, H.Q., Tharreau, D. 2004. Resistance

evaluation of some hybrid rice,

conventional early indica and late japonica

rice to Magnaporthe grisea in China.

Scientia Agr Sinica 37: 362–369.

Wang, Z.X., Yano, M., Yamanouchi, U., Iwamoto,

M., Monna, L., Hayasaka, H., Katayose, Y.

and Sasaki, T. 1999. The Pib gene for rice

blast resistance belong to nucleotide binding

and leucine-rich repeat class of plant disease

resistance gene. The Plant J 19: 55-64

Downloads

Published

2012-04-02

Issue

Vol. 4 No. 1 (2011): Jan-June 2011

Section

Research Articles