Shotgun proteomics analysis of fruit sweetness and ripening in Nam-Dokmai mango (Mangifera indica L.) peel
DOI:
https://doi.org/10.14456/gag.2017.8Keywords:
Sugar content, HPLC, GeLC-MS, mangoAbstract
Nam-Dokmai mango (Mangifera indica) is an important economic crop in Thailand because of its high sweetness and good smell. In order to get the suitable time for fruit harvesting, sugar content in mango flesh and sweetness related proteome in mango peel during ripening were investigated. Mango fruits were collected and divided into 8 developmental stages (15, 30, 45, 60, 75, 90, 105 and 120 days after flowering (DAF)). The sugar content of mango flesh was monitored by means of HPLC. Glucose and fructose were found in the initial stages (15 DAF), while sucrose began to appear since 30 DAF and obviously increased from 105 DAF to 120 DAF (31.20±0.05 µg/mg to 141.52±0.11 µg/mg). Protein profiles in mango peel determined by shotgun proteomics showed that several proteins were related to sugar content in flesh, including proteins playing role in cell metabolic process, cellular component organization or biogenesis, localization, response to stimulus, and developmental process. Association between sweetness relative sugars, fruit ripening related hormones and proteins obtained from mango peel including acetyl-CoA carboxylase, chalcone synthase, NADP-specific isocitrate dehydrogenase and flavin-containing monooxygenase were observed. These proteins might be used to develop as biomarker for mango fruit harvesting.
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