Dietary Phospholipid-Supplementation Affects Blood Metrics, Reproductive Indices, and Biochemical Parameters of Female Shark Catfish, Pangasius nasutus
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Abstract
Dietary phospholipids are known to enhance fish reproductive performance, but their effects on female shark catfish (Pangasius nasutus) remain underexplored. This study investigated the impact of dietary phospholipid supplementation on growth, reproductive performance, and biochemical parameters of female P. nasutus. The experiment used four cages (4×4×3 m) in an earthen pond, each housing 15 fish with an initial average weight of 643±0.34 g and length of 43.3±0.12 cm. Fish were fed a commercial diet supplemented with 0% (control), 1.5%, 2%, or 2.5% phospholipids for 90 days. Results indicated that phospholipid supplementation significantly improved the gonadosomatic index, oocyte development, and steroid hormone levels. The 2.5% phospholipid group exhibited the highest vitellogenin and 17β-estradiol levels, corresponding to advanced oocyte maturation. Hematological parameters, blood biochemistry, and fatty acid composition were unaffected, and no significant improvements in somatic growth or nutrient utilization were observed. The highest supplementation level (2.5%) yielded the greatest increases in the GSI, vitellogenin, and 17β-estradiol levels, resulting in the most developed oocytes. These findings demonstrate that dietary phospholipid supplementation, particularly at 2.5%, effectively enhances broodstock quality and reproductive potential in female P. nasutus.
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