Effect of Cratoxylum formosum Dyer Extract on Sperm Motility and Concentration in L-NAME Hypertensive Rats

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อนุสรณ์ เปาะสะเกษ
เทอดไทย ทองอุ่น
วรรณภา อิชิดะ
ปาริฉัตร ประจะเนย์
พัชรวิภา มณีไสย
ประภัสสร โพธิ์ตึ
พวงรัตน์ ภักดีโชติ


Background and Objective:  Cratoxylum formosum (CF) or Taew Kaao has been reported to have strong antioxidant and anti-inflammatory properties. Little information regarding the effect of CF on blood pressure and sperm quality is demonstrated. This study proposed to evaluate the effect of CF extract on blood pressure, sperm quality and oxidative stress in L-NAME-induced hypertensive rats.

Methods: Male Sprague-Dawley rats were divided into 3 groups (8/group); control, L-NAME and hypertensive treated with CF extract 100 mg/kg BW/day (CF100) groups. Hypertensive group, rats were orally treated with L-NAME 40 mg/kg BW/day in their drinking water to induce hypertension for 5 weeks. In the last 2 weeks, rats in CF100 group were orally treated with CF extract at dose 100 mg/kg BW. At the end of study, systolic blood pressure (SBP), body, testes and epididymis weights were measured. Sperm counting was performed.

Results:  L-NAME treated rats significantly increased SPB, and decreased sperm motility and concentration (p<0.05). Additionally, increased vascular superoxide production was found in L-NAME treated rats (p<0.05), indicating increased oxidative stress in this animal model. There is no significant difference in body weight, testis and epididymis weight among experimental groups. CF extract alleviated the alterations of sperm quality and oxidative stress in hypertensive rats.

Conclusion: This study suggests that L-NAME induced hypertension affects sperm motility and sperm concentration. This might be related to decreased blood flow to the reproductive organs and increased vascular reactive oxygen species production. CF extract can alleviate these alterations via its antioxidant property.


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Sowers JR, Epstein M. Diabetes mellitus and associated hypertension, vascular disease, and nephropathy. An update. Hypertension 1995; 26: 869-79.
2. Kizhakekuttu TJ, Widlansky ME. Natural antioxidants and hypertension: promise and challenges. Cardiovasc Ther 2010; 28: e20-32.
3. Dusing R. Effect of the angiotensin II antagonist valsartan on sexual function in hypertensive men. Blood Press Suppl 2003; 2: 29-34.
4. Fogari R, Zoppi A, Preti P, Rinaldi A, Marasi G, Vanasia A, et al. Sexual activity and plasma testosterone levels in hypertensive males. Am J Hypertens 2002; 15: 217-21.
5. Vlachopoulos C, Ioakeimidis N, Terentes-Printzios D, Aznaouridis K, Rokkas K, Aggelis A, et al. Plasma total testosterone and incident cardiovascular events in hypertensive patients. Am J Hypertens 2013; 26: 373-81.
6. Bunbupha S, Pakdeechote P, Kukongviriyapan U, Prachaney P, Kukongviriyapan V. Asiatic acid reduces blood pressure by enhancing nitric oxide bioavailability with modulation of eNOS and p47phox expression in L-NAME-induced hypertensive rats. Phytother Res 2014; 28: 1506-12.
7. Nakmareong S, Kukongviriyapan U, Pakdeechote P, Donpunha W, Kukongviriyapan V, Kongyingyoes B, et al. Antioxidant and vascular protective effects of curcumin and tetrahydrocurcumin in rats with L-NAME-induced hypertension. Naunyn Schmiedebergs Arch Pharmacol 2011; 383: 519-29.
8. Akinyemi AJ, Adedara IA, Thome GR, Morsch VM, Rovani MT, Mujica LKS, et al. Dietary supplementation of ginger and turmeric improves reproductive function in hypertensive male rats. Toxicol Rep 2015; 2: 1357-66.
9. Nakahara K, Trakoontivakorn G, Alzoreky NS, Ono H, Onishi-Kameyama M, Yoshida M. Antimutagenicity of some edible Thai plants, and a bioactive carbazole alkaloid, mahanine, isolated from Micromelum minutum. J Agric Food Chem 2002; 50: 4796-802.
10. Panthong A, Kanjanapothi D, Taesotikul T, Taylor WC. Ethnobotanical review of medicinal plants from Thai traditional books, Part II: Plants with antidiarrheal, laxative and carminative properties. J Ethnopharmacol 1991; 31: 121-56.
11. Trakoontivakorn G, Nakahara K, Shinmoto H, Takenaka M, Onishi-Kameyama M, Ono H, et al. Structural analysis of a novel antimutagenic compound, 4-Hydroxypanduratin A, and the antimutagenic activity of flavonoids in a Thai spice, fingerroot (Boesenbergia pandurata Schult.) against mutagenic heterocyclic amines. J Agric Food Chem 2001; 49: 3046-50.
12. Kukongviriyapan U, Luangaram S, Leekhaosoong K, Kukongviriyapan V, Preeprame S. Antioxidant and vascular protective activities of Cratoxylum formosum, Syzygium gratum and Limnophila aromatica. Biol Pharm Bull 2007; 30: 661-6.
13. Senggunprai L, Thammaniwit W, Kukongviriyapan V, Prawan A, Kaewseejan N, Siriamornpun S. Cratoxylum formosum extracts inhibit growth and metastasis of cholangiocarcinoma cells by modulating the NF-kappaB and STAT3 pathways. Nutr Cancer 2016; 68: 328-41.
14. Lu FJ, Lin JT, Wang HP, Huang WC. A simple, sensitive, non-stimulated photon counting system for detection of superoxide anion in whole blood. Experientia 1996; 52: 141-4.
15. Arnal JF, Warin L, Michel JB. Determinants of aortic cyclic guanosine monophosphate in hypertension induced by chronic inhibition of nitric oxide synthase. J Clin Invest 1992; 90: 647-52.
16. Gardiner SM, Compton AM, Bennett T, Palmer RM, Moncada S. Control of regional blood flow by endothelium-derived nitric oxide. Hypertension 1990; 15: 486-92.
17. Maneesai P, Prasarttong P, Bunbupha S, Kukongviriyapan U, Kukongviriyapan V, Tangsucharit P, et al. Synergistic antihypertensive effect of Carthamus tinctorius L. extract and captopril in L-NAME-induced hypertensive rats via restoration of eNOS and AT(1)R expression. Nutrients 2016; 8: 122.
18. Xia Y, Zweier JL. Superoxide and peroxynitrite generation from inducible nitric oxide synthase in macrophages. Proc Natl Acad Sci U S A 1997; 94: 6954-8.
19. Adams ML, Nock B, Truong R, Cicero TJ. Nitric oxide control of steroidogenesis: endocrine effects of NG-nitro-L-arginine and comparisons to alcohol. Life Sci 1992; 50: PL35-40.
20. Kim SW, Cuong TD, Hung TM, Ryoo S, Lee JH, Min BS. Arginase II inhibitory activity of flavonoid compounds from Scutellaria indica. Arch Pharm Res 2013; 36: 922-6.