Effect of Cratoxylum formosum Dyer Extract on Sperm Motility and Concentration in L-NAME Hypertensive Rats

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อนุสรณ์ เปาะสะเกษ
เทอดไทย ทองอุ่น
วรรณภา อิชิดะ
ปาริฉัตร ประจะเนย์
พัชรวิภา มณีไสย
ประภัสสร โพธิ์ตึ
พวงรัตน์ ภักดีโชติ

Abstract

Background and Objective:  Cratoxylum formosum (CF) or Taew Kaao has been reported to have strong antioxidant and anti-inflammatory properties. Little information regarding the effect of CF on blood pressure and sperm quality is demonstrated. This study proposed to evaluate the effect of CF extract on blood pressure, sperm quality and oxidative stress in L-NAME-induced hypertensive rats.


Methods: Male Sprague-Dawley rats were divided into 3 groups (8/group); control, L-NAME and hypertensive treated with CF extract 100 mg/kg BW/day (CF100) groups. Hypertensive group, rats were orally treated with L-NAME 40 mg/kg BW/day in their drinking water to induce hypertension for 5 weeks. In the last 2 weeks, rats in CF100 group were orally treated with CF extract at dose 100 mg/kg BW. At the end of study, systolic blood pressure (SBP), body, testes and epididymis weights were measured. Sperm counting was performed.


Results:  L-NAME treated rats significantly increased SPB, and decreased sperm motility and concentration (p<0.05). Additionally, increased vascular superoxide production was found in L-NAME treated rats (p<0.05), indicating increased oxidative stress in this animal model. There is no significant difference in body weight, testis and epididymis weight among experimental groups. CF extract alleviated the alterations of sperm quality and oxidative stress in hypertensive rats.


Conclusion: This study suggests that L-NAME induced hypertension affects sperm motility and sperm concentration. This might be related to decreased blood flow to the reproductive organs and increased vascular reactive oxygen species production. CF extract can alleviate these alterations via its antioxidant property.

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