Effect of Hesperidin on Memory Impairments Induced by Valproic acid in Rats

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Soraya Kaewngam
Tanaporn Anosri
Ram Prajit
Kornrawee Suwannakot
Nataya Sritawan
Anusara Aranarochana
Apiwat Sirichoat1
Wanassanan Pannangrong
Jariya Umka Welbat


Background and objective: Decreasing of neurogenesis in the subgranular zone (SGZ) of the dentate gyrus (DG) in the hippocampus is linked to memory deficits. In animal studies, treatment with valproic acid (VPA) impairs neurogenesis in the SGZ resulting in memory impairment. Hesperidin (Hsd), a plant flavanone, is a natural extract, which enhances learning and memory. Therefore, the aim of this study was to investigate the effect of Hsd on memory impairment in rats induced by VPA.

Methods: Male Sprague Dawley rats were divided into 4 groups (6 animals/group) including vehicle, VPA, Hsd and VPA+Hsd groups. VPA (300 mg/kg) was administrated by intraperitoneal (i.p.) injection twice daily for 14 days. Hsd (100 mg/kg/day) was administrated by oral gavage once a day for 21 days. Body weight was weighed and recorded every day. Three days after the treatment, rats were tested for memory using the novel object location (NOL) and novel object recognition (NOR) tests.

Results: The results showed that the body weight and total exploration time were not significantly different among groups in both NOL and NOR tests (p>0.05). In the NOL test, rats in the vehicle, Hsd and VPA+Hsd groups could significantly discriminate between the novel and familiar locations (p<0.05). In the NOR test, similarly, rats in the vehicle, Hsd and VPA+Hsd groups could significantly discriminate between the novel and familiar objects (p<0.05). In contrast, rats in the VPA group could not significantly performed in both tests.

Conclusion: This study demonstrates that hesperidin could improve the memory impairments induced by valproic acid.


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Kaewngam S, Anosri T, Prajit R, Suwannakot K, Sritawan N, Aranarochana A, Sirichoat1 A, Pannangrong W, Umka Welbat J. Effect of Hesperidin on Memory Impairments Induced by Valproic acid in Rats. SRIMEDJ [Internet]. 2021 Apr. 7 [cited 2023 Jan. 27];36(2):142-8. Available from: https://li01.tci-thaijo.org/index.php/SRIMEDJ/article/view/249574
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