Effects of Limonin on Vascular Function and Morphology in a Rat Model of Obesity

Authors

  • Banyaphon Chanao Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, 40002.
  • Thanapat Sararat Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, 40002
  • Petcharat Chiangsaen Department of Physiology Division, Preclinical Health Science Center, Faculty of Medicine, Bangkokthonburi University, Bangkok, Thailand, 10170
  • Sophida Phuthong Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, 40002.
  • Poungrat Pakdeechote Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, 40002.
  • Putcharawipa Maneesai Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, 40002

Keywords:

limoin, vascular function and morphology, obesity, oxidative stress

Abstract

Background and Objectives: Obesity has been reported to be associated with vascular dysfunction and morphology changes. This study aimed to evaluate the effect of limonin on vascular function and morphology in high fat (HF) diet-induced obesity in rats.

Methods: Male Sprague-Dawley rats were divided into 3 groups; control group fed with normal diet and tap water; obese group fed with a HF-diet and 15% fructose solution for 16 weeks and obese+LM100 group fed with a HF-diet plus 15% fructose solution, and limonin (100 mg/kg/day) for the last 4 weeks. At the end of the study, body weight (BW), retroperitoneal fat weight (RFW), vascular function, vascular morphology and oxidative stress markers were determined.

Results: Obese rats had increases in BW and RFW while treatment with limonin can attenuate the high BW and RFW (p<0.05).  Limonin improved vascular function by reducing the enhancement of contractile response to electrical filed stimulation in the mesenteric vascular beds and attenuated the impairment of vasorelaxation response to acetylcholine in aortic rings isolated from obese rats (p<0.05).  Aortic hypertrophy, indicated by increases in cross-sectional area, aortic wall thickness and wall/lumen ratio, was normalized in obese rats after limonin treatment. Moreover, increased vascular superoxide production, and decreased plasma nitric oxide metabolites were also observed in obese rats and these were restored by limonin treatment (p<0.05).

Conclusion: Limonin alleviated vascular dysfunction and hypertrophy in obese rats. The possible mechanism might associate with its anti-obesity and antioxidant properties.

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Published

2022-04-28

How to Cite

1.
Chanao B, Sararat T, Chiangsaen P, Phuthong S, Pakdeechote P, Maneesai P. Effects of Limonin on Vascular Function and Morphology in a Rat Model of Obesity . SRIMEDJ [Internet]. 2022 Apr. 28 [cited 2024 Oct. 4];37(2):173-8. Available from: https://li01.tci-thaijo.org/index.php/SRIMEDJ/article/view/253741

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