Prunus Domestica L. Crude Extract Protects against Reductions of Memory in D-Galactose-Induced Aging in Adult Rat
Keywords:
memory, Prunus domestica L., D-galactose, agingAbstract
Background and Objective: A decline in memory is the most common pathology of aging. Brain degeneration during aging is related to upregulating the reactive oxygen species (ROS) levels, which induce oxidative stress, neuronal damage, and neuronal apoptosis. In animal studies, D-galactose caused down-regulation of neuronal circuitry and neurogenesis in the subgranular zone (SGZ) of the hippocampal dentate gyrus (DG) resulting in memory impairment. Prunus domestica L. (PD) or look-nai is a fruit that contains phenolic compounds and flavonoids. Previous studies have reported the beneficial effects of PD extract on learning and memory. Therefore, this study aimed to study the effect of PD extract on memory impairment in aging rats induced by D-gal.
Methods: Male Sprague Dawley rats were divided into 8 groups: Vehicle, D-gal, PD 75, PD 100, PD 150, D-gal + PD 75, D-gal + PD 100, and D-gal + PD 150. D-gal (50 mg/kg) and PD (75, 100 and 150 mg/kg) were given by intraperitoneal injection and digestive gavage once a day for 8 weeks, respectively. Rats was weighed during the experiment. Then, memory was tested using the novel object location (NOL) and novel object recognition (NOR) tests.
Result: There were no statistically significant differences (p>0.05) in body weight and locomotor activity among the groups. For the NOL and NOR memory tests, the vehicle, PD 75, PD 100, PD 150, D-gal + PD 75, D-gal + PD 100, and D-gal + PD 150 groups were able to discriminate between the objects placed at the familiar and novel locations or the familiar and novel objects (p<0.05). On the other hand, the D-gal group was unable to distinguish in both tests.
Conclusion: This study demonstrates that PD (75, 100 and 150 mg/kg) had a beneficial effect to ameliorate D-gal-induced memory impairment.
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