ปริมาณฟีโนลิกรวม และฤทธิ์ต้านอนุมูลอิสระของสารสกัดจากส่วนต่าง ๆ ของกล้วยหอมทอง และปัจจัยการสกัดที่เกี่ยวข้อง
DOI:
https://doi.org/10.69598/tbps.14.2.47-60คำสำคัญ:
กล้วยหอมทอง, ปริมาณฟีโนลิกรวม, ฤทธิ์ต้านอนุมูลอิสระ, FRAP, DPPH, ABTSบทคัดย่อ
กล้วยหอมทองเป็นผลไม้ที่นิยมบริโภคส่วนผล และส่วนอื่น ๆ สามารถนำมาใช้ประโยชน์ทางยาได้ ประกอบด้วยสารออกฤทธิ์ทางชีวภาพที่สำคัญในกลุ่มฟีโนลิกและฟลาโวนอยด์ ซึ่งมีฤทธิ์ต้านอนุมูลอิสระ และต้านเชื้อ จุลชีพที่ดี การศึกษานี้มีวัตถุประสงค์เพื่อศึกษาปริมาณฟีโนลิกรวมและฤทธิ์ต้านอนุมูลอิสระของสารสกัดที่ได้จากการสกัดส่วนต่าง ๆ ของกล้วยหอมทอง โดยหาสภาวะการสกัดและส่วนของกล้วยที่ให้ปริมาณฟีโนลิกรวมและฤทธิ์ต้านอนุมูลอิสระจากการทดสอบด้วยวิธี FRAP, DPPH และ ABTS ส่วนต่าง ๆ ของกล้วยหอมทองที่นำมาศึกษาได้แก่ ใบกล้วย ก้านใบ ต้นกล้วย ก้านเครือ เปลือกกล้วยสุก และเปลือกกล้วยดิบ จากการศึกษาเบื้องต้น ส่วนของกล้วยที่มีปริมาณฟีโนลิกรวมสูงสุดคือใบกล้วยและเปลือกกล้วยดิบ จึงนำไปศึกษาเปรียบเทียบสภาวะการสกัดต่าง ๆ ต่อปริมาณฟีโนลิกรวมและฤทธิ์ต้านอนุมูลอิสระ พบว่าสภาวะการสกัดที่ให้ปริมาณฟีโนลิกรวมและฤทธิ์ต้านอนุมูลอิสระสูงสุดคือการสกัดด้วยเอทานอล 70% ที่อุณหภูมิ 55 องศาเซลเซียส เป็นเวลา 4 ชั่วโมง สารสกัดเปลือกกล้วยดิบให้ปริมาณฟีโนลิกรวม 55.94 mgGAE/g และฤทธิ์ต้านอนุมูลอิสระเท่ากับ 59.58, 138.63 และ 276.60 mgAE/g เมื่อทดสอบฤทธิ์ต้านอนุมูลอิสระด้วยวิธี FRAP, DPPH และ ABTS ตามลำดับ ซึ่งสูงกว่าสารสกัดจากใบกล้วย จึงสรุปได้ว่าสารสกัดเปลือกกล้วยดิบมีฤทธิ์ต้านอนุมูลอิสระที่ดีและเหมาะสมที่จะนำไปพัฒนาต่อไป
References
1. Silayoi B. Kluay. Bangkok: kasetsart university press; 2002.
2. Kumar KS, Bhowmik D, Umadevi SDM. Traditional and medicinal uses of banana. J Pharmacog Phytochem. 2012;1(3):51-63.
3. Vu HT, Christopher SJ, Quan VV. Phenolic compounds within banana peel and their potential uses: A review. J Funct Foods. 2018;40:238-48.
4. Anuj Y, Rewa K, Ashwani Y, Mishra JP, Seweta S, Shashi P. Antioxidants and its functions in human body - A Review. Res Environ Life Sci.
2016;9(11):1328-31.
5. Douglas WW, Paul N, Harpal Singh B, Keith G, Ram S, Fabien DM, et al. The Role of Food Antioxidants, Benefits of Functional Foods, and Influence of Feeding Habits on the Health of the Older Person: An Overview. Antioxidants. 2017;6(4):81.
6. Hitoshi M. Role of antioxidants in the skin: Anti-aging effects. J Dermatol Sci. 2010;58(2):85-90.
7. Fatemeh SR, Saifullah R, Abbas FMA, Azhar ME. Total phenolics, flavonoids and antioxidant activity of banana pulp and peel flours: influence of variety and stage of ripeness. Int Food Res J. 2012;19(3):1041-6.
8. Chauhan A, Nagar A, Bala K, Sharma Y. Comparative study of different parts of fruits of Musa Sp. on the basis of their antioxidant activity. Der Pharmacia Lettre. 2016;8(15):88-100.
9. Thaiphanit S, Anprung P. Physicochemical and flavor changes of fragrant banana (musa acuminata aaa group "gross michel") during ripening. J Food Proces Pres. 2009;34(3):366-82.
10. Karuppiah P, Mustaffa M. Antibacterial and antioxidant activities of Musa sp. leaf extracts against multidrug resistant clinical pathogens causing nosocomial infection. Asian Pac J Trop Biomed. 2013;3(9):737-42.
11. Sahaa RK, Acharyaa S, Shovon SSH, Royb P. Medicinal activities of the leaves of Musa sapientum var. sylvesteris in vitro. Asian Pac J Trop Biomed. 2013;3(6):476-82.
12. Dikshit P, Shukla K, Tyagi MK, Garg P, Gambhir JK, Shukla R. Antidiabetic and antihyperlipidemic effects of the stem of Musa sapientum Linn. in streptozotocin-induced diabetic rats. J Diabetes. 2012;4(4):378-85.
13. Navghare VV, Dhawale SC. In vitro antioxidant, hypoglycemic and oral glucose tolerance test of banana peels. Alexandria J Med. 2017;53(3):237-43.
14. Murthy SSN, Felicia C. Antidiabetic activity of Musa sapientum fruit peel on STZ induced diabetic rats. Int J Pharma Bio Sci. 2015;6(1):537-43.
15. Vijai Lakshmi SK, Jamal Akhtar Ansari A, Ali Mahdi A, Kumar Srivastava A. Antidiabetic potential of Musa paradisiaca in Streptozotocin- induced diabetic rats. J Phytopharmacol. 2014;3(2):77-81.
16. Kappel VD, Cazarolli LH, Pereira DF, Postal BG, Madoglio FA, Buss FS, et al. Beneficial effects of banana leaves (Musa x paradisiaca) on glucose homeostasis: multiple sites of action. Braz J Pharmacog. 2013;23(4):706-15.
17. Silvestre MP. Hypoglycemic potential of banana leaves (Musa paradisiaca) in albino rats. Int J Food Eng. 2016;2(1):71-4.
18. Piyush D, Kumar TM, Kirtikar S, Jasvindar GK, Rimi S. Antihypercholesterolemic and antioxidant effect of sterol rich methanol extract of stem of Musa sapientum (banana) in cholesterol fed wistar rats. J Food Sci Technol. 2016;53(3):1690-7.
19. Mallick C, Maiti R, Ghosh D. Comparative study on antihyperglycemic and antihyperlipidemic effects of separate and composite extract of seed of Eugenia jambolana and root of Musa paradisiaca in streptozotocin-induced diabetic male albino rat. Iran J Pharmacol Ther. 2006;5:27-33.
20. Yoo DS, Jeon JM, Choi MJ, Lee HS, Cheon JW, Kim SH, et al. Potential anti-wrinkle effects of m. spaientum l. leaves extract. BioEvolution. 2015;2(2):56-61.
21. Dae SY, Su JJ, Yun JP, Seung HK, Hyung-seo H. Antiaging effects of Musa sapientum L. (banana) leaf extract. J Soc Cosmet Sci Korea. 2016;31:126-34.
22. Hyung SH, Dae SY, Joong HS. Whitening effect of banana leaf extract. J Soc Cosmet Sci Korea. 2016;42:37-43.
23. Toh PY, Leong FS, Chang SK, Khoo HE, Yim HS. Optimization of extraction parameters on the antioxidant properties of banana waste. . Acta Sci Pol Technol Aliment. 2016;15(1):65-78.
24. Katsampa P, Valsamedou E, Grigorakis S, Makris DP. A green ultrasound-assisted extraction process for the recovery of antioxidant polyphenols and pigments from onion solid wastes using Box-Behnken experimental design and kinetics. Industrial Crops and Products. 2015;77:535-43.
25. Rebaya A, Belghith SI, Baghdikian B, Leddet VM, Mabrouki F, Olivier E, et al. Total phenolic, total flavonoid, tannin content, and antioxidant capacity of halimium halimifolium (Cistaceae). J Appl Pharm Sci. 2015;5(1):52-7.
26. Chuenchom P, Swatsitang P, Senawong T, Jogloy S. Antioxidant capacity and phenolic content evaluation on peanut skins from 3 peanut types. Chiang Mai J Sci. 2016;43(1):123-37.
27. Fan R, Li N, Jiang X, Yuan F, Gao Y. HPLC–DAD–MS/MS identification and HPLC–ABTS•+ on-line antioxidant activity evaluation of bioactive compounds in liquorice (Glycyrrhiza uralensis Fisch.) extract. Eur Food Res Technol. 2015;240(5):1035-48.
28. Tan ES, Hamid MK, Aminah A, Mohamad M, Maaruf AG. Antioxidant properties of three banana cultivars (Musa acuminata ‘Berangan’, ‘Mas’ and ‘Raja’) extracts. Sains Malaysiana. 2012;41:319-24.
29. Rafaela G, Lobo GM, Mónica G. Antioxidant activity in banana peel extracts: Testing extraction conditions and related bioactive compounds. Food Chem. 2010;119(3):1030-9.
30. Edelmira V, Ramon V, Francisco G. Inhibition of grape polyphenol oxidase by several natural aliphatic alcohols. J Agric Food Chem. 1990;38(4):1097-100.
31. Renard MGC, Aude WA, Carine LB. Interactions between polyphenols and polysaccharides: Mechanisms and consequences in food processing and digestion. Trends Food Sci Technol. 2017;60:43-51.
32. Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Hawkins Byrne D. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compos Anal. 2006;19(6-7):669-75.
33. Shah P, Modi HA. Comparative study of DPPH, ABTS and FRAP assays for determination of antioxidant activity. Int J Res Appl Sci Eng Technol. 2015;3(6):636-41.
34. Zulueta A, Esteve MJ, Frígola A. ORAC and TEAC assays comparison to measure the antioxidant capacity of food products. Food Chem. 2009;114(1):310-6.
2. Kumar KS, Bhowmik D, Umadevi SDM. Traditional and medicinal uses of banana. J Pharmacog Phytochem. 2012;1(3):51-63.
3. Vu HT, Christopher SJ, Quan VV. Phenolic compounds within banana peel and their potential uses: A review. J Funct Foods. 2018;40:238-48.
4. Anuj Y, Rewa K, Ashwani Y, Mishra JP, Seweta S, Shashi P. Antioxidants and its functions in human body - A Review. Res Environ Life Sci.
2016;9(11):1328-31.
5. Douglas WW, Paul N, Harpal Singh B, Keith G, Ram S, Fabien DM, et al. The Role of Food Antioxidants, Benefits of Functional Foods, and Influence of Feeding Habits on the Health of the Older Person: An Overview. Antioxidants. 2017;6(4):81.
6. Hitoshi M. Role of antioxidants in the skin: Anti-aging effects. J Dermatol Sci. 2010;58(2):85-90.
7. Fatemeh SR, Saifullah R, Abbas FMA, Azhar ME. Total phenolics, flavonoids and antioxidant activity of banana pulp and peel flours: influence of variety and stage of ripeness. Int Food Res J. 2012;19(3):1041-6.
8. Chauhan A, Nagar A, Bala K, Sharma Y. Comparative study of different parts of fruits of Musa Sp. on the basis of their antioxidant activity. Der Pharmacia Lettre. 2016;8(15):88-100.
9. Thaiphanit S, Anprung P. Physicochemical and flavor changes of fragrant banana (musa acuminata aaa group "gross michel") during ripening. J Food Proces Pres. 2009;34(3):366-82.
10. Karuppiah P, Mustaffa M. Antibacterial and antioxidant activities of Musa sp. leaf extracts against multidrug resistant clinical pathogens causing nosocomial infection. Asian Pac J Trop Biomed. 2013;3(9):737-42.
11. Sahaa RK, Acharyaa S, Shovon SSH, Royb P. Medicinal activities of the leaves of Musa sapientum var. sylvesteris in vitro. Asian Pac J Trop Biomed. 2013;3(6):476-82.
12. Dikshit P, Shukla K, Tyagi MK, Garg P, Gambhir JK, Shukla R. Antidiabetic and antihyperlipidemic effects of the stem of Musa sapientum Linn. in streptozotocin-induced diabetic rats. J Diabetes. 2012;4(4):378-85.
13. Navghare VV, Dhawale SC. In vitro antioxidant, hypoglycemic and oral glucose tolerance test of banana peels. Alexandria J Med. 2017;53(3):237-43.
14. Murthy SSN, Felicia C. Antidiabetic activity of Musa sapientum fruit peel on STZ induced diabetic rats. Int J Pharma Bio Sci. 2015;6(1):537-43.
15. Vijai Lakshmi SK, Jamal Akhtar Ansari A, Ali Mahdi A, Kumar Srivastava A. Antidiabetic potential of Musa paradisiaca in Streptozotocin- induced diabetic rats. J Phytopharmacol. 2014;3(2):77-81.
16. Kappel VD, Cazarolli LH, Pereira DF, Postal BG, Madoglio FA, Buss FS, et al. Beneficial effects of banana leaves (Musa x paradisiaca) on glucose homeostasis: multiple sites of action. Braz J Pharmacog. 2013;23(4):706-15.
17. Silvestre MP. Hypoglycemic potential of banana leaves (Musa paradisiaca) in albino rats. Int J Food Eng. 2016;2(1):71-4.
18. Piyush D, Kumar TM, Kirtikar S, Jasvindar GK, Rimi S. Antihypercholesterolemic and antioxidant effect of sterol rich methanol extract of stem of Musa sapientum (banana) in cholesterol fed wistar rats. J Food Sci Technol. 2016;53(3):1690-7.
19. Mallick C, Maiti R, Ghosh D. Comparative study on antihyperglycemic and antihyperlipidemic effects of separate and composite extract of seed of Eugenia jambolana and root of Musa paradisiaca in streptozotocin-induced diabetic male albino rat. Iran J Pharmacol Ther. 2006;5:27-33.
20. Yoo DS, Jeon JM, Choi MJ, Lee HS, Cheon JW, Kim SH, et al. Potential anti-wrinkle effects of m. spaientum l. leaves extract. BioEvolution. 2015;2(2):56-61.
21. Dae SY, Su JJ, Yun JP, Seung HK, Hyung-seo H. Antiaging effects of Musa sapientum L. (banana) leaf extract. J Soc Cosmet Sci Korea. 2016;31:126-34.
22. Hyung SH, Dae SY, Joong HS. Whitening effect of banana leaf extract. J Soc Cosmet Sci Korea. 2016;42:37-43.
23. Toh PY, Leong FS, Chang SK, Khoo HE, Yim HS. Optimization of extraction parameters on the antioxidant properties of banana waste. . Acta Sci Pol Technol Aliment. 2016;15(1):65-78.
24. Katsampa P, Valsamedou E, Grigorakis S, Makris DP. A green ultrasound-assisted extraction process for the recovery of antioxidant polyphenols and pigments from onion solid wastes using Box-Behnken experimental design and kinetics. Industrial Crops and Products. 2015;77:535-43.
25. Rebaya A, Belghith SI, Baghdikian B, Leddet VM, Mabrouki F, Olivier E, et al. Total phenolic, total flavonoid, tannin content, and antioxidant capacity of halimium halimifolium (Cistaceae). J Appl Pharm Sci. 2015;5(1):52-7.
26. Chuenchom P, Swatsitang P, Senawong T, Jogloy S. Antioxidant capacity and phenolic content evaluation on peanut skins from 3 peanut types. Chiang Mai J Sci. 2016;43(1):123-37.
27. Fan R, Li N, Jiang X, Yuan F, Gao Y. HPLC–DAD–MS/MS identification and HPLC–ABTS•+ on-line antioxidant activity evaluation of bioactive compounds in liquorice (Glycyrrhiza uralensis Fisch.) extract. Eur Food Res Technol. 2015;240(5):1035-48.
28. Tan ES, Hamid MK, Aminah A, Mohamad M, Maaruf AG. Antioxidant properties of three banana cultivars (Musa acuminata ‘Berangan’, ‘Mas’ and ‘Raja’) extracts. Sains Malaysiana. 2012;41:319-24.
29. Rafaela G, Lobo GM, Mónica G. Antioxidant activity in banana peel extracts: Testing extraction conditions and related bioactive compounds. Food Chem. 2010;119(3):1030-9.
30. Edelmira V, Ramon V, Francisco G. Inhibition of grape polyphenol oxidase by several natural aliphatic alcohols. J Agric Food Chem. 1990;38(4):1097-100.
31. Renard MGC, Aude WA, Carine LB. Interactions between polyphenols and polysaccharides: Mechanisms and consequences in food processing and digestion. Trends Food Sci Technol. 2017;60:43-51.
32. Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Hawkins Byrne D. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compos Anal. 2006;19(6-7):669-75.
33. Shah P, Modi HA. Comparative study of DPPH, ABTS and FRAP assays for determination of antioxidant activity. Int J Res Appl Sci Eng Technol. 2015;3(6):636-41.
34. Zulueta A, Esteve MJ, Frígola A. ORAC and TEAC assays comparison to measure the antioxidant capacity of food products. Food Chem. 2009;114(1):310-6.
Downloads
เผยแพร่แล้ว
2019-05-01
How to Cite
Charoenteeraboon, J., Ngawhirunpat, T., Chaidedgumjorn, A., Asavapichayont, P., Mahadlek, J., & Tuntarawongsa, S. (2019). ปริมาณฟีโนลิกรวม และฤทธิ์ต้านอนุมูลอิสระของสารสกัดจากส่วนต่าง ๆ ของกล้วยหอมทอง และปัจจัยการสกัดที่เกี่ยวข้อง. ไทยไภษัชยนิพนธ์, 14(2), 47–60. https://doi.org/10.69598/tbps.14.2.47-60
ฉบับ
บท
Original Research Articles
License
All articles published and information contained in this journal such as text, graphics, logos and images is copyrighted by and proprietary to the Thai Bulletin of Pharmaceutical Sciences, and may not be reproduced in whole or in part by persons, organizations, or corporations other than the Thai Bulletin of Pharmaceutical Sciences and the authors without prior written permission.
