Genistein Attenuated Severity of Acute Pancreatitis Induced by L-Arginine in Mice

Authors

  • Jumlongluk Sriko
  • Duangporn Werawatganon
  • Naruemon Klaikaew
  • Prasong Siriviriyakul

Abstract

The roles of genistein on acute pancreatitis are not yet clear. This study was conducted to determine the effects of genistein on anti-inflammatory and improved histopathology of acute pancreatitis in mice. ICR mice were divided into 3 groups (n = 6, each). Control group (Con): Mice received intraperitoneal (i.p.) injection of dimethyl sulfoxide (DMSO) once daily for 4 days; Acute pancreatitis group (AP): mice received two i.p. injection of 350 mg/100 g BW of L-arginine dissolved in normal saline, at an interval of 1 hour. Genistein group (Gen): Mice received 100 mg/kg genistein in 2% DMSO by i.p. injection 2 hours before induction with L-arginine and once daily for 3 days. Mice were sacrificed 72 hours after L-arginine-induced acute pancreatitis. Body weight change, serum amylase, serum interleukin-6 (IL-6), serum C-reactive protein (CRP) and histopathology score were collected. Results: Levels of body weight significantly decreased in AP when compared with Con (AP vs. Con: -1.46 ± 0.37 vs. 1.75 ± 0.19 g). Furthermore, serum amylase, serum IL-6, and serum CRP significantly increased in AP when compared with Con group (AP vs. Con: 13,860.00 ± 5,918.26 vs. 5,714.00 ± 201.11 U/l, 124.68 ± 106.27 vs. 18.59 ± 18.90 pg/ml, and 11,687.07 ± 3,691.95 vs. 8,068.63 ± 3,065.24 ng/ml, respectively; P < 0.05). Genistein resulted in significantly increased levels of body weight compared with AP (Gen vs. AP: 0.41 ± 0.54 vs. -1.46 ± 0.37g). In addition, Gen group had significantly decreased serum amylase, serum IL-6, and serum CRP compared with AP group (Gen vs. AP: 8,728.33 ± 3,213.61 vs. 13,860.00 ± 5,918.26 U/l, 52.58 ± 42.70 vs.124.68 ± 106.27 pg/ml, and 7,607.77 ± 2,757.94 ng/ml vs. 11,687.07 ± 3,691.95 ng/ml, respectively; P < 0.05). Moreover, Gen group showed improved inflammation and histopathology scores. 

Author Biographies

Jumlongluk Sriko

Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

Duangporn Werawatganon

Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

Naruemon Klaikaew

Department of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

Prasong Siriviriyakul

Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

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Published

2018-05-08

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Original Articles