Genistein Attenuated Severity of Acute Pancreatitis Induced by L-Arginine in Mice

Abstract

The roles of genistein on acute pancreatitis are not yet clear. This study was conducted to determine the effects of genistein on anti-inflammatory and improved histopathology of acute pancreatitis in mice. ICR mice were divided into 3 groups (n = 6, each). Control group (Con): Mice received intraperitoneal (i.p.) injection of dimethyl sulfoxide (DMSO) once daily for 4 days; Acute pancreatitis group (AP): mice received two i.p. injection of 350 mg/100 g BW of L-arginine dissolved in normal saline, at an interval of 1 hour. Genistein group (Gen): Mice received 100 mg/kg genistein in 2% DMSO by i.p. injection 2 hours before induction with L-arginine and once daily for 3 days. Mice were sacrificed 72 hours after L-arginine-induced acute pancreatitis. Body weight change, serum amylase, serum interleukin-6 (IL-6), serum C-reactive protein (CRP) and histopathology score were collected. Results: Levels of body weight significantly decreased in AP when compared with Con (AP vs. Con: -1.46 ± 0.37 vs. 1.75 ± 0.19 g). Furthermore, serum amylase, serum IL-6, and serum CRP significantly increased in AP when compared with Con group (AP vs. Con: 13,860.00 ± 5,918.26 vs. 5,714.00 ± 201.11 U/l, 124.68 ± 106.27 vs. 18.59 ± 18.90 pg/ml, and 11,687.07 ± 3,691.95 vs. 8,068.63 ± 3,065.24 ng/ml, respectively; P < 0.05). Genistein resulted in significantly increased levels of body weight compared with AP (Gen vs. AP: 0.41 ± 0.54 vs. -1.46 ± 0.37g). In addition, Gen group had significantly decreased serum amylase, serum IL-6, and serum CRP compared with AP group (Gen vs. AP: 8,728.33 ± 3,213.61 vs. 13,860.00 ± 5,918.26 U/l, 52.58 ± 42.70 vs.124.68 ± 106.27 pg/ml, and 7,607.77 ± 2,757.94 ng/ml vs. 11,687.07 ± 3,691.95 ng/ml, respectively; P < 0.05). Moreover, Gen group showed improved inflammation and histopathology scores.