In Vitro Cell Culture Study of Anticholesterol and Antioxidant Activities of Myrmecodia platytyrae (MyP) Extract in WRL-68 Cells

Abstract

In this cell culture study, the experiments were done in order to explain the efficacy of Myrmecodia platytyrae (MyP) water extract to reduce cholesterol production and its antioxidant effect. The experiment was
performed by measuring total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL) and high-density lipoprotein (HDL) cholesterol levels in the cytosol of the treated WRL-68 cells. In addition, lipid peroxidation was determined by evaluating the concentration of malondialdehyde (MDA) in the cell. The antioxidant effect was also determined by observing superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activities in the treated cell. The results showed that treatment with MyP can decrease TG and LDL concentrations. It was also showed that 400 μg/ml MyP increased HDL concentration. Besides, the levels of MDA were gradually decreased when MyP concentration was increased. Treatment of MyP can also increase SOD and CAT. However, GPx concentration did not increase after MyP treatment. It was concluded that MyP could reduce cholesterol levels and increase SOD and CAT activities in a concentration-dependent manner.