Acute and Subchronic Treatment of Hibiscus sabdariffa Linn. Extract on Renal Function and Lipid Peroxidation in Cisplatin-Induced Acute Renal Failure Rats

Abstract

Acute renal failure (ARF) and an increased renal lipid peroxidation have been found after cisplatin treatment which may be due to an increase in free radical. Hibiscus sabdariffa Linn. (HS) extract has been shown to possess antioxidant effect both in vitro and in vivo, but never been shown to have renal protective effect in ARF rat caused by cisplatin. This study investigated the protective effect of HS water extract (HSE) in ARF rat caused by cisplatin using renal clearance and renal lipid peroxidation study. The experiments were designed as acute and subchronic treatment. Cisplatin (7.5 mg/kg, i.p.) was used to induce ARF. In acute treatment, HSE (250 mg/kg) was orally administered twice; one day and 10 minutes before cisplatin injection. In subchronic treatment, HSE was given daily for seven days before and three days after cisplatin injection. It was found that the acute treatment with HSE was able to attenuate cisplatin-induced ARF by improving both renal plasma flow and glomerular filtration rate and reducing renal malondialdehyde level. The renoprotective effect of HSE may be due to its free radical scavenging property. The toxicity from long term treatment of HSE should be aware of.