Kaempferia parviflora Extract Diminishes Hyperglycemia and Visceral Fat Accumulation in Mice Fed with High Fat and High Sucrose Diet

Abstract

High fat and high sucrose (HFHS) diet are a nutritional condition that accounts for the largest incidence of metabolic syndrome (MetS) which comprises important risk factors that increase the incidence of cardiovascular disease and type 2 diabetes mellitus. Kaempferia parviflora extract (KPE) has been widely used for health promotion as a traditional medicine for improvement of blood flow and as an anti-inflammatory agent. However, there is little work investigation the effect of KPE on MetS. Therefore, the aim of this study was to investigate the effects of KPE on the reduction of body weight, blood glucose concentration and visceral fat accumulation induced by high fat and high sucrose diet in mice. C57BL/6Mlac mice were divided into 6 groups: Control (C), C+KPE10, C+KPE100, HFHS-C, HFHS+KPE10 and HFHS+KPE100. The HFHS groups were given a high fat and high sucrose diet while the C groups received normal diet for 20 weeks, after which they were further treated with KPE 10 or 100 mg/kg BW or distilled water for 8 weeks. Food intake and body weight were measured by weighing, fasting blood glucose was determined by using the glucose oxidase method and blood pressure was measured by tail cuff method. After the animals were sacrificed visceral fat was removed for weighing. The results showed that KPE treatment decreased blood glucose in the HFHS+KPE10 and HFHS+KPE100 groups and reduced visceral fat in the HFHS+KPE10 group when compared with the HFHS-C group. KPE treatment did not alter body weight in all groups. Blood pressure in all groups was not significantly different. In conclusion, KPE treatment is able to reduce symptoms found in high fat and high sucrose diet-induced mice by suppressing visceral fat accumulation and decreasing hyperglycemia.